A CreER mouse to study melanin concentrating hormone signaling in the developing brain

dc.contributor.authorEngle, Staci E.
dc.contributor.authorAntonellis, Patrick J.
dc.contributor.authorWhitehouse, Logan S.
dc.contributor.authorBansal, Ruchi
dc.contributor.authorEmond, Michelle R.
dc.contributor.authorJontes, James D.
dc.contributor.authorKesterson, Robert A.
dc.contributor.authorMykytyn, Kirk
dc.contributor.authorBerbari, Nicolas F.
dc.contributor.departmentBiology, School of Scienceen_US
dc.date.accessioned2019-10-01T16:43:07Z
dc.date.available2019-10-01T16:43:07Z
dc.date.issued2018-08
dc.description.abstractThe neuropeptide, melanin concentrating hormone (MCH), and its G protein-coupled receptor, melanin concentrating hormone receptor 1 (Mchr1), are expressed centrally in adult rodents. MCH signaling has been implicated in diverse behaviors such as feeding, sleep, anxiety, as well as addiction and reward. While a model utilizing the Mchr1 promoter to drive constitutive expression of Cre recombinase (Mchr1-Cre) exists, there is a need for an inducible Mchr1-Cre to determine the roles for this signaling pathway in neural development and adult neuronal function. Here, we generated a BAC transgenic mouse where the Mchr1 promotor drives expression of tamoxifen inducible CreER recombinase. Many aspects of the Mchr1-Cre expression pattern are recapitulated by the Mchr1-CreER model, though there are also notable differences. Most strikingly, compared to the constitutive model, the new Mchr1-CreER model shows strong expression in adult animals in hypothalamic brain regions involved in feeding behavior but diminished expression in regions involved in reward, such as the nucleus accumbens. The inducible Mchr1-CreER allele will help reveal the potential for Mchr1 signaling to impact neural development and subsequent behavioral phenotypes, as well as contribute to the understanding of the MCH signaling pathway in terminally differentiated adult neurons and the diverse behaviors that it influences.en_US
dc.identifier.citationEngle, S. E., Antonellis, P. J., Whitehouse, L. S., Bansal, R., Emond, M. R., Jontes, J. D., … Berbari, N. F. (2018). A CreER mouse to study melanin concentrating hormone signaling in the developing brain. Genesis (New York, N.Y. : 2000), 56(8), e23217. doi:10.1002/dvg.23217en_US
dc.identifier.urihttps://hdl.handle.net/1805/21006
dc.language.isoen_USen_US
dc.publisherWileyen_US
dc.relation.isversionof10.1002/dvg.23217en_US
dc.relation.journalGenesisen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.sourcePMCen_US
dc.subjectArcuate nucleusen_US
dc.subjectHypothalamusen_US
dc.subjectMedian eminenceen_US
dc.subjectNucleus accumbensen_US
dc.subjectParaventricular nucleusen_US
dc.titleA CreER mouse to study melanin concentrating hormone signaling in the developing brainen_US
dc.typeArticleen_US
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