Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections
| dc.contributor.author | Mlodzianoski, Michael J. | |
| dc.contributor.author | Cheng-Hathaway, Paul J. | |
| dc.contributor.author | Bemiller, Shane M. | |
| dc.contributor.author | McCray, Tyler J. | |
| dc.contributor.author | Liu, Sheng | |
| dc.contributor.author | Miller, David A. | |
| dc.contributor.author | Lamb, Bruce T. | |
| dc.contributor.author | Landreth, Gary E. | |
| dc.contributor.author | Huang, Fang | |
| dc.contributor.department | Anatomy and Cell Biology, IU School of Medicine | en_US |
| dc.date.accessioned | 2019-07-01T20:18:03Z | |
| dc.date.available | 2019-07-01T20:18:03Z | |
| dc.date.issued | 2018-08 | |
| dc.description.abstract | Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-μm-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-β filaments in a mouse model of Alzheimer's disease. | en_US |
| dc.eprint.version | Author's manuscript | en_US |
| dc.identifier.citation | Mlodzianoski, M. J., Cheng-Hathaway, P. J., Bemiller, S. M., McCray, T. J., Liu, S., Miller, D. A., … Huang, F. (2018). Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections. Nature methods, 15(8), 583–586. doi:10.1038/s41592-018-0053-8 | en_US |
| dc.identifier.uri | https://hdl.handle.net/1805/19790 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Springer Nature | en_US |
| dc.relation.isversionof | 10.1038/s41592-018-0053-8 | en_US |
| dc.relation.journal | Nature Methods | en_US |
| dc.rights | Publisher Policy | en_US |
| dc.source | PMC | en_US |
| dc.subject | Alzheimer Disease | en_US |
| dc.subject | Membrane Glycoproteins | en_US |
| dc.subject | Plaque -- Amyloid | en_US |
| dc.subject | Receptors -- Immunologic | en_US |
| dc.title | Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections | en_US |
| dc.type | Article | en_US |