Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections
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2018-08
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American English
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Springer Nature
Abstract
Application of single-molecule switching nanoscopy (SMSN) beyond the coverslip surface poses substantial challenges due to sample-induced aberrations that distort and blur single-molecule emission patterns. We combined active shaping of point spread functions and efficient adaptive optics to enable robust 3D-SMSN imaging within tissues. This development allowed us to image through 30-μm-thick brain sections to visualize and reconstruct the morphology and the nanoscale details of amyloid-β filaments in a mouse model of Alzheimer's disease.
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Mlodzianoski, M. J., Cheng-Hathaway, P. J., Bemiller, S. M., McCray, T. J., Liu, S., Miller, D. A., … Huang, F. (2018). Active PSF shaping and adaptive optics enable volumetric localization microscopy through brain sections. Nature methods, 15(8), 583–586. doi:10.1038/s41592-018-0053-8
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Nature Methods
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PMC
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