Quantification of murine myocardial infarct size using 2-D and 4-D high-frequency ultrasound

dc.contributor.authorDann, Melissa M.
dc.contributor.authorClark, Sydney Q.
dc.contributor.authorTrzaskalski, Natasha A.
dc.contributor.authorEarl, Conner C.
dc.contributor.authorSchepers, Luke E.
dc.contributor.authorPulente, Selena M.
dc.contributor.authorLennord, Ebonee N.
dc.contributor.authorAnnamalai, Karthik
dc.contributor.authorGruber, Joseph M.
dc.contributor.authorCox, Abigail D.
dc.contributor.authorLorenzen-Schmidt, Ilka
dc.contributor.authorSeymour, Richard
dc.contributor.authorKim, Kyoung-Han
dc.contributor.authorGoergen, Craig J.
dc.contributor.authorMulvihill, Erin E.
dc.contributor.departmentMedicine, School of Medicine
dc.date.accessioned2023-10-31T14:04:47Z
dc.date.available2023-10-31T14:04:47Z
dc.date.issued2022
dc.description.abstractIschemic heart disease is the leading cause of death in the United States, Canada, and worldwide. Severe disease is characterized by coronary artery occlusion, loss of blood flow to the myocardium, and necrosis of tissue, with subsequent remodeling of the heart wall, including fibrotic scarring. The current study aims to demonstrate the efficacy of quantitating infarct size via two-dimensional (2-D) echocardiographic akinetic length and four-dimensional (4-D) echocardiographic infarct volume and surface area as in vivo analysis techniques. We further describe and evaluate a new surface area strain analysis technique for estimating myocardial infarction (MI) size after ischemic injury. Experimental MI was induced in mice via left coronary artery ligation. Ejection fraction and infarct size were measured through 2-D and 4-D echocardiography. Infarct size established via histology was compared with ultrasound-based metrics via linear regression analysis. Two-dimensional echocardiographic akinetic length (r = 0.76, P = 0.03), 4-D echocardiographic infarct volume (r = 0.85, P = 0.008), and surface area (r = 0.90, P = 0.002) correlate well with histology. Although both 2-D and 4-D echocardiography were reliable measurement techniques to assess infarct, 4-D analysis is superior in assessing asymmetry of the left ventricle and the infarct. Strain analysis performed on 4-D data also provides additional infarct sizing techniques, which correlate with histology (surface strain: r = 0.94, P < 0.001, transmural thickness: r = 0.76, P = 0.001). Two-dimensional echocardiographic akinetic length, 4-D echocardiography ultrasound, and strain provide effective in vivo methods for measuring fibrotic scarring after MI. NEW & NOTEWORTHY: Our study supports that both 2-D and 4-D echocardiographic analysis techniques are reliable in quantifying infarct size though 4-D ultrasound provides a more holistic image of LV function and structure, especially after myocardial infarction. Furthermore, 4-D strain analysis correctly identifies infarct size and regional LV dysfunction after MI. Therefore, these techniques can improve functional insight into the impact of pharmacological interventions on the pathophysiology of cardiac disease.
dc.identifier.citationDann MM, Clark SQ, Trzaskalski NA, et al. Quantification of murine myocardial infarct size using 2-D and 4-D high-frequency ultrasound. Am J Physiol Heart Circ Physiol. 2022;322(3):H359-H372. doi:10.1152/ajpheart.00476.2021
dc.identifier.urihttps://hdl.handle.net/1805/36808
dc.language.isoen_US
dc.publisherAmerican Physiological Society
dc.relation.isversionof10.1152/ajpheart.00476.2021
dc.relation.journalAmerican Journal of Physiology: Heart and Circulatory Physiology
dc.rightsPublisher Policy
dc.sourcePMC
dc.subjectEchocardiography
dc.subjectInfarct size
dc.subjectMouse models
dc.subjectStrain
dc.subjectUltrasound
dc.titleQuantification of murine myocardial infarct size using 2-D and 4-D high-frequency ultrasound
dc.typeArticle
ul.alternative.fulltexthttps://www.ncbi.nlm.nih.gov/pmc/articles/PMC8836752/
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