Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique
| dc.contributor.author | Cadar, Adrian G. | |
| dc.contributor.author | Feaster, Tromondae K. | |
| dc.contributor.author | Durbin, Matthew D. | |
| dc.contributor.author | Hong, Charles C. | |
| dc.contributor.department | Pediatrics, School of Medicine | en_US |
| dc.date.accessioned | 2019-05-06T18:31:45Z | |
| dc.date.available | 2019-05-06T18:31:45Z | |
| dc.date.issued | 2017-08-14 | |
| dc.description.abstract | This unit describes the published Matrigel mattress method. Briefly, we describe the preparation of the mattress, replating of the human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) on the Matrigel mattress, and hiPSC-CM mattress maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC-CMs, which can be used for downstream applications. | en_US |
| dc.eprint.version | Author's manuscript | en_US |
| dc.identifier.citation | Cadar, A. G., Feaster, T. K., Durbin, M. D., & Hong, C. C. (2017). Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique. Current protocols in stem cell biology, 42, 4A.14.1–4A.14.7. doi:10.1002/cpsc.32 | en_US |
| dc.identifier.uri | https://hdl.handle.net/1805/19136 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Wiley | en_US |
| dc.relation.isversionof | 10.1002/cpsc.32 | en_US |
| dc.relation.journal | Current Protocols in Stem Cell Biology | en_US |
| dc.rights | Publisher Policy | en_US |
| dc.source | PMC | en_US |
| dc.subject | Matrigel | en_US |
| dc.subject | Cardiac | en_US |
| dc.subject | hiPSC-CMs | en_US |
| dc.subject | Myocytes | en_US |
| dc.subject | Stem cells | en_US |
| dc.title | Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique | en_US |
| dc.type | Article | en_US |