Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique

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2017-08-14
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American English
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Wiley
Abstract

This unit describes the published Matrigel mattress method. Briefly, we describe the preparation of the mattress, replating of the human induced pluripotent stem cell-derived cardiomyocytes (hiPSC-CM) on the Matrigel mattress, and hiPSC-CM mattress maintenance. Adherence to this protocol will yield individual, robustly shortening hiPSC-CMs, which can be used for downstream applications.

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Cadar, A. G., Feaster, T. K., Durbin, M. D., & Hong, C. C. (2017). Production of Single Contracting Human Induced Pluripotent Stem Cell-Derived Cardiomyocytes: Matrigel Mattress Technique. Current protocols in stem cell biology, 42, 4A.14.1–4A.14.7. doi:10.1002/cpsc.32
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Current Protocols in Stem Cell Biology
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PMC
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