Protein expression profiles of intestinal epithelial co-cultures: effect of functionalised carbon nanotube exposure

dc.contributor.authorLai, Xianyin
dc.contributor.authorBlazer-Yost, Bonnie L.
dc.contributor.authorClack, James W.
dc.contributor.authorFears, Sharry L.
dc.contributor.authorMitra, Somenath
dc.contributor.authorNtim, Susana Addo
dc.contributor.authorRingham, Heather N.
dc.contributor.authorWitzmann, Frank A.
dc.contributor.departmentCellular and Integrative Physiology, School of Medicine
dc.date.accessioned2025-04-28T09:50:07Z
dc.date.available2025-04-28T09:50:07Z
dc.date.issued2013
dc.description.abstractTo assess the biological effects of low level, water dispersible, functionalised carbon nanotube (f-CNT) exposure in an in vitro model simulating the digestive tract, cellular protein expression was quantified and compared using label-free quantitative mass spectrometry (LFQMS). Co-cultured cells were exposed to well-characterised SWCNT-COOH, MWCNT-COOH, and MWCNT-PVP. The relative expression of 2,282 unique proteins was compared across the dose groups. 428 proteins were found to be differentially expressed. At the high dose, the extent of differential protein expression was CNT-specific and directly related to CNT colloidal stability. Cells responded to low level MWCNT-PVP exposure with three-fold greater differential expression. Bioinformatic analysis indicated significant and f-CNT-specific effects on relevant molecular and cellular functions and canonical pathways, with little overlap across f-CNT type and in the absence of overt toxicity.
dc.eprint.versionAuthor's manuscript
dc.identifier.citationLai X, Blazer-Yost BL, Clack JW, et al. Protein expression profiles of intestinal epithelial co-cultures: effect of functionalised carbon nanotube exposure. Int J Biomed Nanosci Nanotechnol. 2013;3(1-2):10.1504/IJBNN.2013.054508. doi:10.1504/IJBNN.2013.054508
dc.identifier.urihttps://hdl.handle.net/1805/47495
dc.language.isoen_US
dc.publisherInderscience
dc.relation.isversionof10.1504/IJBNN.2013.054508
dc.relation.journalInternational Journal of Biomedical Nanoscience and Nanotechnology
dc.rightsPublisher Policy
dc.sourcePMC
dc.subjectCarbon nanotube
dc.subjectColon
dc.subjectIn vitro
dc.subjectIntestinal cells
dc.subjectLabel-free quantitation
dc.subjectMass spectrometry
dc.subjectProteomics
dc.titleProtein expression profiles of intestinal epithelial co-cultures: effect of functionalised carbon nanotube exposure
dc.typeArticle
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