Class I histone deacetylases in retinal progenitors and differentiating ganglion cells

dc.contributor.authorSaha, Ankita
dc.contributor.authorTiwari, Sarika
dc.contributor.authorDharmarajan, Subramanian
dc.contributor.authorOtteson, Deborah C.
dc.contributor.authorBelecky-Adams, Teri L.
dc.contributor.departmentBiology, School of Scienceen_US
dc.date.accessioned2018-10-12T14:35:54Z
dc.date.available2018-10-12T14:35:54Z
dc.date.issued2018-12
dc.description.abstractBackground The acetylation state of histones has been used as an indicator of the developmental state of progenitor and differentiating cells. The goal of this study was to determine the nuclear localization patterns of Class I histone deacetylases (HDACs) in retinal progenitor cells (RPCs) and retinal ganglion cells (RGCs), as the first step in understanding their potential importance in cell fate determination within the murine retina. Results The only HDAC to label RPC nuclei at E16 and P5 was HDAC1. In contrast, there was generally increased nuclear localization of all Class I HDACs in differentiating RGCs. Between P5 and P30, SOX2 expression becomes restricted to Müller glial, cholinergic amacrine cells, and retinal astrocytes. Cholinergic amacrine showed a combination of changes in nuclear localization of Class I HDACs. Strikingly, although Müller glia and retinal astrocytes express many of the same genes, P30 Müller glial cells showed nuclear localization only of HDAC1, while retinal astrocytes were positive for HDACs 1, 2, and 3. Conclusion These results indicate there may be a role for one or more of the Class I HDACs in retinal cell type-specific differentiation.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationSaha, A., Tiwari, S., Dharmarajan, S., Otteson, D. C., & Belecky-Adams, T. L. (2018). Class I histone deacetylases in retinal progenitors and differentiating ganglion cells. Gene Expression Patterns, 30, pp. 37-48. https://doi.org/10.1016/j.gep.2018.08.007en_US
dc.identifier.urihttps://hdl.handle.net/1805/17530
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.gep.2018.08.007en_US
dc.relation.journalGene Expression Patternsen_US
dc.rightsPublisher Policyen_US
dc.sourceAuthoren_US
dc.subjecthistone deacetylasesen_US
dc.subjectHDACsen_US
dc.subjectmouseen_US
dc.titleClass I histone deacetylases in retinal progenitors and differentiating ganglion cellsen_US
dc.typeArticleen_US
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