Genetic dissection of triplicated chromosome 21 orthologs yields varying skeletal traits in Down syndrome model mice

dc.contributor.authorSloan, Kourtney
dc.contributor.authorThomas, Jared
dc.contributor.authorBlackwell, Matthew
dc.contributor.authorVoisard, Deanna
dc.contributor.authorLana-Elola, Eva
dc.contributor.authorWatson-Scales, Sheona
dc.contributor.authorRoper, Daniel L.
dc.contributor.authorWallace, Joseph M.
dc.contributor.authorFisher, Elizabeth M. C.
dc.contributor.authorTybulewicz, Victor L. J.
dc.contributor.authorRoper, Randall J.
dc.contributor.departmentBiology, School of Science
dc.date.accessioned2023-12-22T12:44:50Z
dc.date.available2023-12-22T12:44:50Z
dc.date.issued2023
dc.description.abstractDown syndrome (DS) phenotypes result from triplicated genes, but the effects of three copy genes are not well known. A mouse mapping panel genetically dissecting human chromosome 21 (Hsa21) syntenic regions was used to investigate the contributions and interactions of triplicated Hsa21 orthologous genes on mouse chromosome 16 (Mmu16) on skeletal phenotypes. Skeletal structure and mechanical properties were assessed in femurs of male and female Dp9Tyb, Dp2Tyb, Dp3Tyb, Dp4Tyb, Dp5Tyb, Dp6Tyb, Ts1Rhr and Dp1Tyb;Dyrk1a+/+/− mice. Dp1Tyb mice, with the entire Hsa21 homologous region of Mmu16 triplicated, display bone deficits similar to those of humans with DS and served as a baseline for other strains in the panel. Bone phenotypes varied based on triplicated gene content, sex and bone compartment. Three copies of Dyrk1a played a sex-specific, essential role in trabecular deficits and may interact with other genes to influence cortical deficits related to DS. Triplicated genes in Dp9Tyb and Dp2Tyb mice improved some skeletal parameters. As triplicated genes can both improve and worsen bone deficits, it is important to understand the interaction between and molecular mechanisms of skeletal alterations affected by these genes.
dc.identifier.citationSloan K, Thomas J, Blackwell M, et al. Genetic dissection of triplicated chromosome 21 orthologs yields varying skeletal traits in Down syndrome model mice. Dis Model Mech. 2023;16(4):dmm049927. doi:10.1242/dmm.049927
dc.identifier.urihttps://hdl.handle.net/1805/37511
dc.language.isoen_US
dc.publisherThe Company of Biologists
dc.relation.isversionof10.1242/dmm.049927
dc.relation.journalDisease Models & Mechanisms
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourcePMC
dc.subjectDown syndrome
dc.subjectTrisomy 21
dc.subjectSkeletal deficits
dc.subjectAnimal models
dc.subjectGenetics
dc.titleGenetic dissection of triplicated chromosome 21 orthologs yields varying skeletal traits in Down syndrome model mice
dc.typeArticle
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