Deep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopy

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dc.contributor.authorClendenon, Sherry G.
dc.contributor.authorYoung, Pamela A.
dc.contributor.authorFerkowicz, Michael
dc.contributor.authorPhillips, Carrie
dc.contributor.authorDunn, Kenneth W.
dc.contributor.departmentDepartment of Pediatrics, IU School of Medicineen_US
dc.date.accessioned2016-02-22T16:26:14Z
dc.date.available2016-02-22T16:26:14Z
dc.date.issued2011-08
dc.description.abstractIn scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering. We applied the concept of clearing to deep tissue imaging of highly scattering specimens. Clearing is a remarkably effective approach to improving image quality at depth using either confocal or multiphoton microscopy. Tissue clearing appears to eliminate the need for multiphoton excitation for deep tissue imaging.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationClendenon, S. G., Young, P. A., Ferkowicz, M., Phillips, C., & Dunn, K. W. (2011). Deep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopy. Microscopy and Microanalysis : The Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 17(4), 614–617. http://doi.org/10.1017/S1431927611000535en_US
dc.identifier.issn1431-9276en_US
dc.identifier.urihttps://hdl.handle.net/1805/8413
dc.language.isoen_USen_US
dc.publisherCambridge University Pressen_US
dc.relation.isversionof10.1017/S1431927611000535en_US
dc.relation.journalMicroscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canadaen_US
dc.rightsPublisher Policyen_US
dc.sourcePMCen_US
dc.subjectFluorescenceen_US
dc.subjectImage Processing, Computer-Assisteden_US
dc.subjectmethodsen_US
dc.subjectKidneyen_US
dc.subjectchemistryen_US
dc.subjectcytologyen_US
dc.subjectMicroscopy, Confocalen_US
dc.titleDeep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopyen_US
dc.typeArticleen_US
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