Deep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopy
If you need an accessible version of this item, please submit a remediation request.
Date
2011-08
Language
American English
Embargo Lift Date
Committee Members
Degree
Degree Year
Department
Grantor
Journal Title
Journal ISSN
Volume Title
Found At
Cambridge University Press
Abstract
In scattering specimens, multiphoton excitation and nondescanned detection improve imaging depth by a factor of 2 or more over confocal microscopy; however, imaging depth is still limited by scattering. We applied the concept of clearing to deep tissue imaging of highly scattering specimens. Clearing is a remarkably effective approach to improving image quality at depth using either confocal or multiphoton microscopy. Tissue clearing appears to eliminate the need for multiphoton excitation for deep tissue imaging.
Description
item.page.description.tableofcontents
item.page.relation.haspart
Cite As
Clendenon, S. G., Young, P. A., Ferkowicz, M., Phillips, C., & Dunn, K. W. (2011). Deep Tissue Fluorescent Imaging in Scattering Specimens Using Confocal Microscopy. Microscopy and Microanalysis : The Official Journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada, 17(4), 614–617. http://doi.org/10.1017/S1431927611000535
ISSN
1431-9276
Publisher
Series/Report
Sponsorship
Major
Extent
Identifier
Relation
Journal
Microscopy and microanalysis : the official journal of Microscopy Society of America, Microbeam Analysis Society, Microscopical Society of Canada
Source
PMC
Alternative Title
Type
Article
Number
Volume
Conference Dates
Conference Host
Conference Location
Conference Name
Conference Panel
Conference Secretariat Location
Permanent Link
Version
Author's manuscript