Estimation of the number of contributors of theoretical mixture profiles based on allele counting: Does increasing the number of loci increase success rate of estimates?

dc.contributor.authorDembinski, Gina M.
dc.contributor.authorSobieralski, Carl
dc.contributor.authorPicard, Christine J.
dc.contributor.departmentBiology, School of Scienceen_US
dc.date.accessioned2017-12-29T15:26:05Z
dc.date.available2017-12-29T15:26:05Z
dc.date.issued2018-03
dc.description.abstractDNA mixtures are more frequently encountered in casework due to increased kit sensitivity, protocols with increased cycle number, and requests for low copy number DNA samples to be tested. Generally, the first step in mixture interpretation is determining the number of contributors, with the most common approach of maximum allele count. Although there are previous studies regarding the accuracy of this approach, none have evaluated the accuracy with the newly expanded U.S. core STR loci. In this work, 4,976,355 theoretical mixture combinations were generated with the PowerPlex® Fusion 6C system which includes 23 autosomal STR loci and three Y-STR loci. The number of contributors could be correctly assumed for 100% two-person and 99.99% three-person mixtures, whereas, four-, five-, and six-person mixtures were correctly assumed in 89.7%, 57.3%, and 7.8% of mixtures, respectively. Y-STR analysis showed the 3 Y-STR markers are only accurate for two-person male mixtures (96.7%). This work demonstrates that maximum allele count using the expanded U.S. core loci is not much improved from previous smaller panels, reiterating that this method is not as accurate beyond three contributors.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationDembinski, G. M., Sobieralski, C., & Picard, C. J. (2018). Estimation of the number of contributors of theoretical mixture profiles based on allele counting: Does increasing the number of loci increase success rate of estimates? Forensic Science International: Genetics, 33, 24-32. https://doi.org/10.1016/j.fsigen.2017.11.007en_US
dc.identifier.urihttps://hdl.handle.net/1805/14925
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.fsigen.2017.11.007en_US
dc.relation.journalForensic Science International: Geneticsen_US
dc.rightsPublisher Policyen_US
dc.sourceAuthoren_US
dc.subjectforensic scienceen_US
dc.subjectDNAen_US
dc.subjectmixturesen_US
dc.titleEstimation of the number of contributors of theoretical mixture profiles based on allele counting: Does increasing the number of loci increase success rate of estimates?en_US
dc.typeArticleen_US
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