miR-1908 as a novel prognosis marker of glioma via promoting malignant phenotype and modulating SPRY4/RAF1 axis

dc.contributor.authorChai, Zhi
dc.contributor.authorFan, Huijie
dc.contributor.authorLi, Yanyan
dc.contributor.authorSong, Lijuan
dc.contributor.authorJin, Xiaoming
dc.contributor.authorYu, Jiezhong
dc.contributor.authorLi, Yanhua
dc.contributor.authorMa, Cungen
dc.contributor.authorZhou, Ran
dc.contributor.departmentMedicine, School of Medicineen_US
dc.date.accessioned2018-07-24T14:14:37Z
dc.date.available2018-07-24T14:14:37Z
dc.date.issued2017-11
dc.description.abstractMicroRNAs (miRNAs) are reported to be involved in the development of glioma. However, study on miRNAs in glioma is limited. The present study aimed to identify miRNAs which can act as potential novel prognostic markers for glioma and analyze its possible mechanism. We show that miR-1908 correlates with shorter survival time of glioma patients via promoting cell proliferation, invasion, anti-apoptosis and regulating SPRY4/RAF1 axis. Analysis of GEO and TCGA database found that miR-1908 was significantly upregulated in glioma tissues, and strongly associated with shorter survival time of glioma patients. Further Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis revealed that miR-1908 is mainly involved in regulating cell proliferation, invasion and apoptosis. To further confirm the above results, in vitro, glioma U251 cells were transfected with miR-1908 mimics or inhibitor, and upregulated miR-1908 promoted U251 cell proliferation, and enhanced the ability of invasion by transwell assay. In addition, upregulated miR-1908 also enhanced anti-apoptosis ability of U251 cells through decreasing pro-apoptosis protein Bax expression. Since miRNAs regulate numerous biological processes by targeting broad set of messenger RNAs, validated target genes of miR-1908 in glioma were analyzed by Targetscan and miRTarBase databases. Among them SPRY4 was significantly decreased in glioma tissues and associated with short survival time, which was selected as the key target gene of miR-1908. Moreover, protein-protein interaction (PPI) showed that SPRY4 could interacted with pro-oncogene RAF1 and negatively correlated with RAF1 expression. Consistent with above analysis, in vitro, western blot analysis identified that miR-1908 upregulated significantly decreased SPRY4 expression and increased RAF1 expression. Hence, miR-1908 was correlated with poor prognosis of glioma via promoting cell proliferation, invasion, anti-apoptosis and regulating SPRF4/RAF1 axis. Our results elucidated the tumor promoting role of miR-1908 and established miR-1908 as a potential novel prognostic marker for glioma.en_US
dc.eprint.versionFinal published versionen_US
dc.identifier.citationChai, Z., Fan, H., Li, Y., Song, L., Jin, X., Yu, J., … Zhou, R. (2017). miR-1908 as a novel prognosis marker of glioma via promoting malignant phenotype and modulating SPRY4/RAF1 axis. Oncology Reports, 38(5), 2717–2726. http://doi.org/10.3892/or.2017.6003en_US
dc.identifier.urihttps://hdl.handle.net/1805/16776
dc.language.isoen_USen_US
dc.publisherSpandidos Publicationsen_US
dc.relation.isversionof10.3892/or.2017.6003en_US
dc.relation.journalOncology Reportsen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/
dc.sourcePMCen_US
dc.subjectmiR-1908en_US
dc.subjectGliomaen_US
dc.subjectPrognostic markeren_US
dc.subjectSPRY4en_US
dc.subjectRAF1en_US
dc.subjectApoptosisen_US
dc.titlemiR-1908 as a novel prognosis marker of glioma via promoting malignant phenotype and modulating SPRY4/RAF1 axisen_US
dc.typeArticleen_US
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