CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice
| dc.contributor.author | Cassidy, Annelise | |
| dc.contributor.author | Pelletier, Stephane | |
| dc.contributor.department | Medical and Molecular Genetics, School of Medicine | |
| dc.date.accessioned | 2023-10-24T11:50:26Z | |
| dc.date.available | 2023-10-24T11:50:26Z | |
| dc.date.issued | 2023 | |
| dc.description.abstract | In this protocol, we describe the generation of conditional alleles in mice using the DECAI (DEgradation based on Cre-regulated Artificial Intron) approach. We detail steps for the CRISPR-mediated insertion of the short DECAI cassette within exon 3 of Scyl1 and the functional validation of alleles at genomic, transcriptomic, and protein levels. This strategy simplifies the process of generating mice with conditional alleles. | |
| dc.eprint.version | Final published version | |
| dc.identifier.citation | Cassidy A, Pelletier S. CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice. STAR Protoc. 2023;4(1):102116. doi:10.1016/j.xpro.2023.102116 | |
| dc.identifier.uri | https://hdl.handle.net/1805/36597 | |
| dc.language.iso | en_US | |
| dc.publisher | Elsevier | |
| dc.relation.isversionof | 10.1016/j.xpro.2023.102116 | |
| dc.relation.journal | STAR Protocols | |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | en |
| dc.rights.uri | https://creativecommons.org/licenses/by-nc-nd/4.0 | |
| dc.source | PMC | |
| dc.subject | Developmental biology | |
| dc.subject | Genetics | |
| dc.subject | Model Organisms | |
| dc.subject | Molecular Biology | |
| dc.subject | CRISPR | |
| dc.title | CRISPR-Cas9-mediated insertion of a short artificial intron for the generation of conditional alleles in mice | |
| dc.type | Article |