Multiscale activity imaging in mammary gland reveals how oxytocin enables lactation

dc.contributor.authorStevenson, Alexander J.
dc.contributor.authorVanwalleghem, Gilles
dc.contributor.authorStewart, Teneale A.
dc.contributor.authorCondon, Nicholas D.
dc.contributor.authorLloyd-Lewis, Bethan
dc.contributor.authorMarino, Natascia
dc.contributor.authorPutney, James W.
dc.contributor.authorScott, Ethan K.
dc.contributor.authorEwing, Adam D.
dc.contributor.authorDavis, Felicity M.
dc.contributor.departmentMedicine, School of Medicineen_US
dc.date.accessioned2020-11-20T18:16:00Z
dc.date.available2020-11-20T18:16:00Z
dc.date.issued2020
dc.description.abstractThe mammary epithelium is indispensable for the continued survival of more than 5000 mammalian species. For some, the volume of milk ejected in a single day exceeds their entire blood volume. Here, we unveil the spatiotemporal properties of physiological signals that orchestrate milk ejection. Using quantitative, multidimensional imaging of mammary cell ensembles, we reveal how stimulus evoked Ca2+ oscillations couple to contractions in basal epithelial cells. Moreover, we show that Ca2+-dependent contractions generate the requisite force to physically deform the innermost layer of luminal cells, compelling them to discharge the fluid that they produced and housed. Through the collective action of thousands of these biological positive displacement pumps, each linked to a contractile ductal network, milk is delivered into the mouth of the dependent neonate, seconds after the command.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationStevenson, A. J., Vanwalleghem, G., Stewart, T. A., Condon, N. D., Lloyd-Lewis, B., Marino, N., Putney, J. W., Scott, E. K., Ewing, A. D., & Davis, F. M. (2020). Multiscale imaging of basal cell dynamics in the functionally mature mammary gland. Proceedings of the National Academy of Sciences, 117(43), 26822–26832. https://doi.org/10.1073/pnas.2016905117en_US
dc.identifier.urihttps://hdl.handle.net/1805/24456
dc.language.isoenen_US
dc.relation.isversionof10.1101/657510en_US
dc.relation.journalProceedings of the National Academy of Sciencesen_US
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 International
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourceOtheren_US
dc.subjectcalcium signalingen_US
dc.subjectgenetically-encoded calcium indicatorsen_US
dc.subjectoscillationsen_US
dc.titleMultiscale activity imaging in mammary gland reveals how oxytocin enables lactationen_US
dc.typeArticleen_US
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