Genome-wide analysis and proteomic studies reveal APE1/Ref-1 multifunctional role in mammalian cells

dc.contributor.authorVascotto, Carlo
dc.contributor.authorCesaratto, Laura
dc.contributor.authorZeef, Leo AH.
dc.contributor.authorDeganuto, Marta
dc.contributor.authorD'Ambrosio, Chiara
dc.contributor.authorScaloni, Andrea
dc.contributor.authorRomanello, Milena
dc.contributor.authorDamante, Giuseppe
dc.contributor.authorTaglialatela, Giulio
dc.contributor.authorDelneri, Daniela
dc.contributor.authorKelley, Mark R.
dc.contributor.authorMitra, Sankar
dc.contributor.authorQuadrifoglio, Franco
dc.contributor.authorTell, Gianluca
dc.date.accessioned2014-07-30T14:01:46Z
dc.date.available2014-07-30T14:01:46Z
dc.date.issued2009-02
dc.description.abstractApurinic apyrimidinic endonuclease/redox effector factor 1 (APE1/Ref-1) protects cells from oxidative stress by acting as a central enzyme in base excision repair pathways of DNA lesions and through its independent activity as a redox transcriptional co-activator. Dysregulation of this protein has been associated with cancer development. At present, contrasting data have been published regarding the biological relevance of the two functions as well as the molecular mechanisms involved. Here, we combined both mRNA expression profiling and proteomic analysis to determine the molecular changes associated with APE1 loss-of-expression induced by siRNA technology. This approach identified a role of APE1 in cell growth, apoptosis, intracellular redox state, mitochondrial function, and cytoskeletal structure. Overall, our data show that APE1 acts as a hub in coordinating different and vital functions in mammalian cells, highlighting the molecular determinants of the multifunctional nature of APE1 protein.en_US
dc.identifier.citationVascotto, C., Cesaratto, L., Zeef, L. A., Deganuto, M., D'Ambrosio, C., Scaloni, A., ... & Tell, G. (2009). Genome‐wide analysis and proteomic studies reveal APE1/Ref‐1 multifunctional role in mammalian cells. Proteomics, 9(4), 1058-1074.en_US
dc.identifier.urihttps://hdl.handle.net/1805/4805
dc.language.isoen_USen_US
dc.subjectAPE1/Ref-1en_US
dc.subjectcell signalingen_US
dc.subjectdifference analysisen_US
dc.titleGenome-wide analysis and proteomic studies reveal APE1/Ref-1 multifunctional role in mammalian cellsen_US
dc.typeArticleen_US
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