Isolate-Dependent Growth, Virulence, and Cell Wall Composition in the Human Pathogen Aspergillus fumigatus
dc.contributor.author | Amarsaikhan, Nansalmaa | |
dc.contributor.author | O’Dea, Evan M. | |
dc.contributor.author | Tsoggerel, Angar | |
dc.contributor.author | Owegi, Henry | |
dc.contributor.author | Gillenwater, Jordan | |
dc.contributor.author | Templeton, Steven P. | |
dc.contributor.department | Microbiology and Immunology, School of Medicine | |
dc.date.accessioned | 2025-04-03T11:08:26Z | |
dc.date.available | 2025-04-03T11:08:26Z | |
dc.date.issued | 2014-06-19 | |
dc.description.abstract | The ubiquitous fungal pathogen Aspergillus fumigatus is a mediator of allergic sensitization and invasive disease in susceptible individuals. The significant genetic and phenotypic variability between and among clinical and environmental isolates are important considerations in host-pathogen studies of A. fumigatus-mediated disease. We observed decreased radial growth, rate of germination, and ability to establish colony growth in a single environmental isolate of A. fumigatus, Af5517, when compared to other clinical and environmental isolates. Af5517 also exhibited increased hyphal diameter and cell wall β-glucan and chitin content, with chitin most significantly increased. Morbidity, mortality, lung fungal burden, and tissue pathology were decreased in neutropenic Af5517-infected mice when compared to the clinical isolate Af293. Our results support previous findings that suggest a correlation between in vitro growth rates and in vivo virulence, and we propose that changes in cell wall composition may contribute to this phenotype. | |
dc.eprint.version | Final published version | |
dc.identifier.citation | Amarsaikhan N, O'Dea EM, Tsoggerel A, Owegi H, Gillenwater J, Templeton SP. Isolate-dependent growth, virulence, and cell wall composition in the human pathogen Aspergillus fumigatus. PLoS One. 2014;9(6):e100430. Published 2014 Jun 19. doi:10.1371/journal.pone.0100430 | |
dc.identifier.uri | https://hdl.handle.net/1805/46783 | |
dc.language.iso | en_US | |
dc.publisher | Public Library of Science | |
dc.relation.isversionof | 10.1371/journal.pone.0100430 | |
dc.relation.journal | PLoS One | |
dc.rights | Attribution 4.0 International | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | PMC | |
dc.subject | Aspergillosis | |
dc.subject | Aspergillus fumigatus | |
dc.subject | Neutropenia | |
dc.subject | Pneumonia | |
dc.title | Isolate-Dependent Growth, Virulence, and Cell Wall Composition in the Human Pathogen Aspergillus fumigatus | |
dc.type | Article |