Digital Light Processing 3D Bioprinting of Gelatin-Norbornene Hydrogel for Enhanced Vascularization

dc.contributor.authorDuong, Van Thuy
dc.contributor.authorLin, Chien-Chi
dc.contributor.departmentBiomedical Engineering, Purdue School of Engineering and Technology
dc.date.accessioned2025-01-22T09:19:45Z
dc.date.available2025-01-22T09:19:45Z
dc.date.issued2023
dc.description.abstractDigital light processing (DLP) bioprinting can be used to fabricate volumetric scaffolds with intricate internal structures, such as perfusable vascular channels. The successful implementation of DLP bioprinting in tissue fabrication requires using suitable photo-reactive bioinks. Norbornene-based bioinks have emerged as an attractive alternative to (meth)acrylated macromers in 3D bioprinting owing to their mild and rapid reaction kinetics, high cytocompatibility for in situ cell encapsulation, and adaptability for post-printing modification or conjugation of bioactive motifs. In this contribution, the development of gelatin-norbornene (GelNB) is reported as a photo-cross-linkable bioink for DLP 3D bioprinting. Low concentrations of GelNB (2-5 wt.%) and poly(ethylene glycol)-tetra-thiol (PEG4SH) are DLP-printed with a wide range of stiffness (G' ≈120 to 4000 Pa) and with perfusable channels. DLP-printed GelNB hydrogels are highly cytocompatible, as demonstrated by the high viability of the encapsulated human umbilical vein endothelial cells (HUVECs). The encapsulated HUVECs formed an interconnected microvascular network with lumen structures. Notably, the GelNB bioink permitted both in situ tethering and secondary conjugation of QK peptide, a vascular endothelial growth factor (VEGF)-mimetic peptide. Incorporation of QK peptide significantly improved endothelialization and vasculogenesis of the DLP-printed GelNB hydrogels, reinforcing the applicability of this bioink system in diverse biofabrication applications.
dc.eprint.versionAuthor's manuscript
dc.identifier.citationDuong VT, Lin CC. Digital Light Processing 3D Bioprinting of Gelatin-Norbornene Hydrogel for Enhanced Vascularization. Macromol Biosci. 2023;23(12):e2300213. doi:10.1002/mabi.202300213
dc.identifier.urihttps://hdl.handle.net/1805/45346
dc.language.isoen_US
dc.publisherWiley
dc.relation.isversionof10.1002/mabi.202300213
dc.relation.journalMacromolecular Bioscience
dc.rightsPublisher Policy
dc.sourcePMC
dc.subjectQK peptides
dc.subjectDigital light processing printing
dc.subjectGelatin-norbornene
dc.subjectSoft gels
dc.subjectVascularization
dc.titleDigital Light Processing 3D Bioprinting of Gelatin-Norbornene Hydrogel for Enhanced Vascularization
dc.typeArticle
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