Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis
| dc.contributor.author | Lin, Yang | |
| dc.contributor.author | Kobayashi, Michihiro | |
| dc.contributor.author | Portilho, Nathalia Azevedo | |
| dc.contributor.author | Mishra, Akansha | |
| dc.contributor.author | Gao, Hongyu | |
| dc.contributor.author | Liu, Yunlong | |
| dc.contributor.author | Wenzel, Pamela | |
| dc.contributor.author | Davis, Brian | |
| dc.contributor.author | Yoder, Mervin C. | |
| dc.contributor.author | Yoshimoto, Momoko | |
| dc.contributor.department | Pediatrics, School of Medicine | en_US |
| dc.date.accessioned | 2019-08-02T14:05:39Z | |
| dc.date.available | 2019-08-02T14:05:39Z | |
| dc.date.issued | 2019-03-05 | |
| dc.description.abstract | It is generally considered that mouse embryonic stem cell (ESC) differentiation into blood cells in vitro recapitulates yolk sac (YS) hematopoiesis. As such, similar to YS-derived B-progenitors, we demonstrate here that ESC-derived B-progenitors differentiate into B-1 and marginal zone B cells, but not B-2 cells in immunodeficient mice after transplantation. ESC-derived B-1 cells were maintained in the recipients for more than 6 months, secreting natural IgM antibodies in vivo. Gene expression profiling displayed a close relationship between ESC- and YS-derived B-1 progenitors. Because there are no hematopoietic stem cells (HSCs) detectable in our ESC differentiation culture, successful long-term engraftment of ESC-derived functional B-1 cells supports the presence of HSC-independent B-1 cell development. | en_US |
| dc.eprint.version | Final published version | en_US |
| dc.identifier.citation | Lin, Y., Kobayashi, M., Azevedo Portilho, N., Mishra, A., Gao, H., Liu, Y., … Yoshimoto, M. (2019). Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis. Stem cell reports, 12(3), 572–583. doi:10.1016/j.stemcr.2019.01.006 | en_US |
| dc.identifier.uri | https://hdl.handle.net/1805/20145 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Elsevier | en_US |
| dc.relation.isversionof | 10.1016/j.stemcr.2019.01.006 | en_US |
| dc.relation.journal | Stem Cell Reports | en_US |
| dc.rights | Attribution-NonCommercial-NoDerivs 3.0 United States | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/3.0/us/ | * |
| dc.source | PMC | en_US |
| dc.subject | B-1 cells | en_US |
| dc.subject | ESCs | en_US |
| dc.subject | HSC-independent lymphopoiesis | en_US |
| dc.subject | Mouse embryo | en_US |
| dc.subject | Yolk sac | en_US |
| dc.title | Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis | en_US |
| dc.type | Article | en_US |