Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis

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dc.contributor.authorLin, Yang
dc.contributor.authorKobayashi, Michihiro
dc.contributor.authorPortilho, Nathalia Azevedo
dc.contributor.authorMishra, Akansha
dc.contributor.authorGao, Hongyu
dc.contributor.authorLiu, Yunlong
dc.contributor.authorWenzel, Pamela
dc.contributor.authorDavis, Brian
dc.contributor.authorYoder, Mervin C.
dc.contributor.authorYoshimoto, Momoko
dc.contributor.departmentPediatrics, School of Medicineen_US
dc.date.accessioned2019-08-02T14:05:39Z
dc.date.available2019-08-02T14:05:39Z
dc.date.issued2019-03-05
dc.description.abstractIt is generally considered that mouse embryonic stem cell (ESC) differentiation into blood cells in vitro recapitulates yolk sac (YS) hematopoiesis. As such, similar to YS-derived B-progenitors, we demonstrate here that ESC-derived B-progenitors differentiate into B-1 and marginal zone B cells, but not B-2 cells in immunodeficient mice after transplantation. ESC-derived B-1 cells were maintained in the recipients for more than 6 months, secreting natural IgM antibodies in vivo. Gene expression profiling displayed a close relationship between ESC- and YS-derived B-1 progenitors. Because there are no hematopoietic stem cells (HSCs) detectable in our ESC differentiation culture, successful long-term engraftment of ESC-derived functional B-1 cells supports the presence of HSC-independent B-1 cell development.en_US
dc.eprint.versionFinal published versionen_US
dc.identifier.citationLin, Y., Kobayashi, M., Azevedo Portilho, N., Mishra, A., Gao, H., Liu, Y., … Yoshimoto, M. (2019). Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis. Stem cell reports, 12(3), 572–583. doi:10.1016/j.stemcr.2019.01.006en_US
dc.identifier.urihttps://hdl.handle.net/1805/20145
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.stemcr.2019.01.006en_US
dc.relation.journalStem Cell Reportsen_US
dc.rightsAttribution-NonCommercial-NoDerivs 3.0 United States*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/3.0/us/*
dc.sourcePMCen_US
dc.subjectB-1 cellsen_US
dc.subjectESCsen_US
dc.subjectHSC-independent lymphopoiesisen_US
dc.subjectMouse embryoen_US
dc.subjectYolk sacen_US
dc.titleLong-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesisen_US
dc.typeArticleen_US
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