Counting Photobleach Steps and the Dynamics of Bacterial Predators

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Date
2016-04-08
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American English
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Office of the Vice Chancellor for Research
Abstract

Photobleach (PB) counting is used to enumerate proteins by monitoring how the light intensity in some regions decreases by quanta as individual fluorophores photobleach. While it is straightforward in theory, PB counting is often difficult because fluorescence traces are noisy. In this work, we quantify the sources of noise that arise during photobleach counting to construct a principled likelihood function of observing the data given a model. Noise in the signal could arise from background fluorescence, variable fluorophore emission, and fluorophore blinking. In addition, in a completely different direction, we explore the role of hydrodynamic interactions on the dynamics of bacterial predators. Our study shows that Bdellovibrio (BV) - a model predatory bacterium - is susceptible to self-generated hydrodynamic forces. Near surfaces and defects, these hydrodynamic interactions co-localize BV with its prey, and this may enhance BV’s hunting efficiency.

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Hossein Jashnsaz, Konstantinos Tsekouras, Mohammed Al Juboori, Corey Weistuch, Nick Miller, Tyler Nguyen, Bryan McCoy, Stephanie Perkins, Bruce Ray, Gregory Anderson, and Steve Presse. 2016, April 8. Counting Photobleach Steps and the Dynamics of Bacterial Predators. Poster session presented at IUPUI Research Day 2016, Indianapolis, Indiana.
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