Physicochemical and Biological Properties of Novel Chlorhexidine-Loaded Nanotube-Modified Dentin Adhesive
dc.contributor.author | Feitosa, Sabrina A. | |
dc.contributor.author | Palasuk, Jadesada | |
dc.contributor.author | Geraldeli, Saulo | |
dc.contributor.author | Windsor, L. Jack | |
dc.contributor.author | Bottino, Marco C. | |
dc.contributor.department | Biomedical Sciences and Comprehensive Care, School of Dentistry | en_US |
dc.date.accessioned | 2020-06-04T17:59:36Z | |
dc.date.available | 2020-06-04T17:59:36Z | |
dc.date.issued | 2018-09-10 | |
dc.description.abstract | A commercially available three-step (etch-and-rinse) adhesive was modified by adding chlorhexidine (CHX)-loaded nanotubes (Halloysite®, HNT) at two concentrations (CHX10% and CHX20%). The experimental groups were: SBMP (unmodified adhesive, control), HNT (SBMP modified with HNT), CHX10 (SBMP modified with HNT loaded with CHX10%), and CHX20 (SBMP modified with HNT loaded with CHX20%). Changes in the degree of conversion (DC%), Knoop hardness (KHN), water sorption (WS), solubility (SL), antimicrobial activity, cytotoxicity, and anti-matrix metalloproteinase [MMP-1] activity (collagenase-I) were evaluated. In regards to DC%, two-way ANOVA followed by Tukey’s post-hoc test revealed that only the factor “adhesive” was statistically significant (p<0.05). No significant differences were detected in DC% when 20 s light-curing was used (p>0.05). For Knoop microhardness, one-way ANOVA followed by the Tukey’s test showed statistically significant differences when comparing HNT (20.82±1.65) and CHX20% (21.71±2.83) with the SBMP and CHX10% groups. All adhesives presented similar WS and cytocompatibility. The CHX-loaded nanotube-modified adhesive released enough CHX to inhibit the growth of S. mutans and L. casei. Adhesive eluates were not able to effectively inhibit MMP-1 activity. The evaluation of higher CHX concentrations might be necessary to provide an effective and predictable MMP inhibition. | en_US |
dc.eprint.version | Author's manuscript | en_US |
dc.identifier.citation | Feitosa, S. A., Palasuk, J., Geraldeli, S., Windsor, L. J., & Bottino, M. C. (2019). Physicochemical and biological properties of novel chlorhexidine-loaded nanotube-modified dentin adhesive. Journal of biomedical materials research. Part B, Applied biomaterials, 107(3), 868–875. https://doi.org/10.1002/jbm.b.34183 | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/22903 | |
dc.language.iso | en_US | en_US |
dc.publisher | Wiley | en_US |
dc.relation.isversionof | 10.1002/jbm.b.34183 | en_US |
dc.relation.journal | Journal of biomedical materials research. Part B, Applied biomaterials | en_US |
dc.rights | Publisher Policy | en_US |
dc.source | PMC | en_US |
dc.subject | Halloysite | en_US |
dc.subject | Nanotubes | en_US |
dc.subject | Chlorhexidine | en_US |
dc.subject | Dentin | en_US |
dc.subject | Adhesive | en_US |
dc.subject | Matrix metalloproteinase | en_US |
dc.title | Physicochemical and Biological Properties of Novel Chlorhexidine-Loaded Nanotube-Modified Dentin Adhesive | en_US |
dc.type | Article | en_US |