Inhibitory Effects of Alpha-Connexin Carboxyl-Terminal Peptide on Canine Mammary Epithelial Cells: A Study on Benign and Malignant Phenotypes
dc.contributor.author | da Fonseca, Ivone Izabel Mackowiak | |
dc.contributor.author | Nagamine, Marcia Kazumi | |
dc.contributor.author | Sato, Ayami | |
dc.contributor.author | Rossatto, Carlos Alberto, Jr. | |
dc.contributor.author | Yeh, Elizabeth Shinmay | |
dc.contributor.author | Dagli, Lucia Zaidan | |
dc.contributor.department | Pharmacology and Toxicology, School of Medicine | |
dc.date.accessioned | 2024-06-11T10:36:59Z | |
dc.date.available | 2024-06-11T10:36:59Z | |
dc.date.issued | 2024-02-18 | |
dc.description.abstract | Mammary cancer is highly prevalent in non-castrated female dogs. Cell-to-cell communication is an important mechanism to maintain homeostasis, and connexins are proteins that assemble to form the communicating gap junctions. In many cancers, communication capacity is reduced; several approaches are being tested in order to increase the communication capacity in cancer cells and, therefore, alter their viability. This study analyzed the effects of the alpha-connexin carboxyl-terminal peptide (αCT1) on canine mammary non-neoplastic and neoplastic epithelial cells. Seven canine epithelial mammary cell lines were used. Among these, one was a normal canine epithelial mammary cell line (LOEC-NMG), two canine mammary adenomas (LOEC-MAd1 and LOEC-MAd2), and four canine mammary adenocarcinomas (LOEC-MCA1, LOEC-MCA2, LOEC-MCA3 and CF41). The αCT1 corresponds to a short Cx43 C-terminal sequence linked to an internalization sequence called the antennapedia. After 24 h of incubation, the medium containing different αCT1 peptide concentrations was added to the cells, and only the culture medium was used for control. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) test was used to quantify cell viability before treatment and 48, 72, and 96 h after the treatment. Results showed that the normal mammary epithelial cell line (LOEC-NMG) was resistant to treatment with αCT1, which is consistent with a previous study on human mammary cell lines. One of the adenoma cell lines (LOEC-MAd2) was also resistant to treatment with αCT1, although the other (LOEC-MAd1) was susceptible to treatment, mostly at 72 h after treatment. Regarding the four canine adenocarcinoma cell lines, they differ regarding the susceptibility to the treatment with αCT1. Three cell lines, canine mixed adenocarcinoma (LOEC-MCA1), canine complex adenocarcinoma (LOEC-MCA2), and commercial canine mammary adenocarcinoma cell line CF41, were susceptible to treatment with αCT1, while one canine mammary adenocarcinoma cell line (LOEC-MCA3) was resistant to treatment. In most αCT1 treated cell lines, Cx43 was strongly detected in cell membranes by immunofluorescence. We propose that αCT1 restored the cell-to-cell communication capacity of neoplastic cells and induced inhibitory effects on cell viability. | |
dc.eprint.version | Final published version | |
dc.identifier.citation | da Fonseca IIM, Nagamine MK, Sato A, Rossatto-Jr CA, Yeh ES, Dagli MLZ. Inhibitory Effects of Alpha-Connexin Carboxyl-Terminal Peptide on Canine Mammary Epithelial Cells: A Study on Benign and Malignant Phenotypes. Cancers (Basel). 2024;16(4):820. Published 2024 Feb 18. doi:10.3390/cancers16040820 | |
dc.identifier.uri | https://hdl.handle.net/1805/41386 | |
dc.language.iso | en_US | |
dc.publisher | MDPI | |
dc.relation.isversionof | 10.3390/cancers16040820 | |
dc.relation.journal | Cancers | |
dc.rights | Attribution 4.0 International | en |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
dc.source | PMC | |
dc.subject | Connexin | |
dc.subject | Mammary cells | |
dc.subject | Canine | |
dc.subject | Viability | |
dc.title | Inhibitory Effects of Alpha-Connexin Carboxyl-Terminal Peptide on Canine Mammary Epithelial Cells: A Study on Benign and Malignant Phenotypes | |
dc.type | Article |