The role of follicular helper T cells and the germinal center in HIV-1 gp120 DNA prime and gp120 protein boost vaccination

dc.contributor.authorHollister, Kristin
dc.contributor.authorChen, Yuxin
dc.contributor.authorWang, Shixia
dc.contributor.authorWu, Hao
dc.contributor.authorMondal, Arpita
dc.contributor.authorClegg, Ninah
dc.contributor.authorLu, Shan
dc.contributor.authorDent, Alexander
dc.contributor.departmentDepartment of Microbiology and Immunology, IU School of Medicineen_US
dc.date.accessioned2016-06-28T19:07:35Z
dc.date.available2016-06-28T19:07:35Z
dc.date.issued2014-07
dc.description.abstractThe importance of follicular T helper (TFH) cells and the germinal center (GC) reaction in the humoral immune response has become clear in recent years, however the role of TFH cells and the GC in an HIV vaccine strategy remains unclear. In this study, we primed mice with gp120-encoding DNA and boosted with gp120 protein, a regimen previously shown to induce high titers of high affinity and cross-reactive anti-gp120 Abs. Priming with gp120 DNA caused increased TFH cell differentiation, GC B cells, and antigen-specific antibody titers, compared with priming with gp120 protein. Priming with DNA also caused more activated CD4(+) T cells to become TFH cells and more GC B cells to become memory cells. Deletion of BCL6 midway through the vaccine regimen resulted in loss of TFH cells and GCs, and, unexpectedly, increased anti-gp120 IgG titers and avidity. Our data suggests vaccination with gp120-encoding DNA elicits a stronger and more rapid TFH and GC response than gp120 protein. Furthermore, we demonstrate that the GC reaction may actually limit antigen-specific IgG secretion in the context of repeated immunizations.en_US
dc.identifier.citationHollister, K., Chen, Y., Wang, S., Wu, H., Mondal, A., Clegg, N., … Dent, A. (2014). The role of follicular helper T cells and the germinal center in HIV-1 gp120 DNA prime and gp120 protein boost vaccination. Human Vaccines & Immunotherapeutics, 10(7), 1985–1992. http://doi.org/10.4161/hv.28659en_US
dc.identifier.urihttps://hdl.handle.net/1805/10227
dc.language.isoen_USen_US
dc.publisherTaylor & Francisen_US
dc.relation.isversionof10.4161/hv.28659en_US
dc.relation.journalHuman Vaccines & Immunotherapeuticsen_US
dc.rightsPublisher Policyen_US
dc.sourcePMCen_US
dc.subjectBCL6en_US
dc.subjectHIV vaccineen_US
dc.subjectFollicular helper T cellsen_US
dc.subjectGerminal centeren_US
dc.subjectPrime-boosten_US
dc.titleThe role of follicular helper T cells and the germinal center in HIV-1 gp120 DNA prime and gp120 protein boost vaccinationen_US
dc.typeArticleen_US
ul.alternative.fulltexthttp://www.ncbi.nlm.nih.gov/pmc/articles/PMC4186047/en_US
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