Three-dimensional nanoscopy of whole cells and tissues with in situ point spread function retrieval
dc.contributor.author | Xu, Fan | |
dc.contributor.author | Ma, Donghan | |
dc.contributor.author | MacPherson, Kathryn P. | |
dc.contributor.author | Liu, Sheng | |
dc.contributor.author | Bu, Ye | |
dc.contributor.author | Wang, Yu | |
dc.contributor.author | Tang, Yu | |
dc.contributor.author | Bi, Cheng | |
dc.contributor.author | Kwok, Tim | |
dc.contributor.author | Chubykin, Alexander A. | |
dc.contributor.author | Yin, Peng | |
dc.contributor.author | Calve, Sarah | |
dc.contributor.author | Landreth, Gary E. | |
dc.contributor.author | Huang, Fang | |
dc.contributor.department | Anatomy and Cell Biology, School of Medicine | en_US |
dc.date.accessioned | 2021-12-13T20:29:02Z | |
dc.date.available | 2021-12-13T20:29:02Z | |
dc.date.issued | 2020-05 | |
dc.description.abstract | Single-molecule localization microscopy is a powerful tool for visualizing subcellular structures, interactions and protein functions in biological research. However, inhomogeneous refractive indices inside cells and tissues distort the fluorescent signal emitted from single-molecule probes, which rapidly degrades resolution with increasing depth. We propose a method that enables the construction of an in situ 3D response of single emitters directly from single-molecule blinking datasets, and therefore allows their locations to be pinpointed with precision that achieves the Cramér-Rao lower bound and uncompromised fidelity. We demonstrate this method, named in situ PSF retrieval (INSPR), across a range of cellular and tissue architectures, from mitochondrial networks and nuclear pores in mammalian cells to amyloid-β plaques and dendrites in brain tissues and elastic fibers in developing cartilage of mice. This advancement expands the routine applicability of super-resolution microscopy from selected cellular targets near coverslips to intra- and extracellular targets deep inside tissues. | en_US |
dc.eprint.version | Author's manuscript | en_US |
dc.identifier.citation | Xu, F., Ma, D., MacPherson, K. P., Liu, S., Bu, Y., Wang, Y., Tang, Y., Bi, C., Kwok, T., Chubykin, A. A., Yin, P., Calve, S., Landreth, G. E., & Huang, F. (2020). Three-dimensional nanoscopy of whole cells and tissues with in situ point spread function retrieval. Nature Methods, 17(5), 531–540. https://doi.org/10.1038/s41592-020-0816-x | en_US |
dc.identifier.issn | 1548-7105 | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/27165 | |
dc.language.iso | en | en_US |
dc.publisher | Nature | en_US |
dc.relation.isversionof | 10.1038/s41592-020-0816-x | en_US |
dc.relation.journal | Nature Methods | en_US |
dc.rights | Publisher Policy | en_US |
dc.source | PMC | en_US |
dc.subject | Animals | en_US |
dc.subject | Cartilage | en_US |
dc.subject | Cell Nucleus | en_US |
dc.title | Three-dimensional nanoscopy of whole cells and tissues with in situ point spread function retrieval | en_US |
dc.type | Article | en_US |
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