In vivo axial loading of the mouse tibia

dc.contributor.authorMelville, Katherine M.
dc.contributor.authorRobling, Alexander G.
dc.contributor.authorvan der Meulen, Marjolein C. H.
dc.contributor.departmentDepartment of Anatomy and Cell Biology, IU School of Medicineen_US
dc.date.accessioned2016-05-31T14:36:13Z
dc.date.available2016-05-31T14:36:13Z
dc.date.issued2015
dc.description.abstractNoninvasive methods to apply controlled, cyclic loads to the living skeleton are used as anabolic procedures to stimulate new bone formation in adults and enhance bone mass accrual in growing animals. These methods are also invaluable for understanding bone signaling pathways. Our focus here is on a particular loading model: in vivo axial compression of the mouse tibia. An advantage of loading the tibia is that changes are present in both the cancellous envelope of the proximal tibia and the cortical bone of the tibial diaphysis. To load the tibia of the mouse axially in vivo, a cyclic compressive load is applied up to five times a week to a single tibia per mouse for a duration lasting from 1 day to 6 weeks. With the contralateral limb as an internal control, the anabolic response of the skeleton to mechanical stimuli can be studied in a pairwise experimental design. Here, we describe the key parameters that must be considered before beginning an in vivo mouse tibial loading experiment, including methods for in vivo strain gauging of the tibial midshaft, and then we describe general methods for loading the mouse tibia for an experiment lasting multiple days.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationMelville, K. M., Robling, A. G., & van der Meulen, M. C. H. (2015). In vivo axial loading of the mouse tibia. Methods in Molecular Biology (Clifton, N.J.), 1226, 99–115. http://doi.org/10.1007/978-1-4939-1619-1_9en_US
dc.identifier.urihttps://hdl.handle.net/1805/9695
dc.publisherSpringeren_US
dc.relation.isversionof10.1007/978-1-4939-1619-1_9en_US
dc.relation.journalMethods in Molecular Biologyen_US
dc.sourcePMCen_US
dc.subjectboneen_US
dc.subjectmechanical loadingen_US
dc.subjecttibiaen_US
dc.subjectmouseen_US
dc.subjectanabolic bone formationen_US
dc.titleIn vivo axial loading of the mouse tibiaen_US
dc.typeArticleen_US
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