Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches

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dc.contributor.authorAcott, Ted S.
dc.contributor.authorFautsch, Michael P.
dc.contributor.authorMao, Weiming
dc.contributor.authorEthier, C. Ross
dc.contributor.authorHuang, Alex S.
dc.contributor.authorKelley, Mary J.
dc.contributor.authorAga, Mini
dc.contributor.authorBhattacharya, Sanjoy K.
dc.contributor.authorBorras, Terete
dc.contributor.authorBovenkamp, Diane
dc.contributor.authorChowdhury, Uttio Roy
dc.contributor.authorClark, Abbot F.
dc.contributor.authorDibas, Mohammed I.
dc.contributor.authorDu, Yiqin
dc.contributor.authorElliott, Michael H.
dc.contributor.authorFaralli, Jennifer A.
dc.contributor.authorGong, Haiyan
dc.contributor.authorHerberg, Samuel
dc.contributor.authorJohnstone, Murray A.
dc.contributor.authorKaufman, Paul L.
dc.contributor.authorKeller, Kate E.
dc.contributor.authorKelly, Ruth A.
dc.contributor.authorKrizaj, David
dc.contributor.authorKuehn, Markus H.
dc.contributor.authorLi, Hoi Lam
dc.contributor.authorLieberman, Raquel
dc.contributor.authorLin, Shan C.
dc.contributor.authorLiu, Yutao
dc.contributor.authorMcDonnell, Fiona S.
dc.contributor.authorMcDowell, Colleen M.
dc.contributor.authorMcLellan, Gillian J.
dc.contributor.authorMzyk, Philip
dc.contributor.authorNair, Kayarat Saidas
dc.contributor.authorOverby, Darryl R.
dc.contributor.authorPeters, Donna M.
dc.contributor.authorRaghunathan, VijayKrishna
dc.contributor.authorRao, Ponugoti Vasantha
dc.contributor.authorRoddy, Gavin W.
dc.contributor.authorSharif, Najam A.
dc.contributor.authorShim, Myoung Sup
dc.contributor.authorSun, Yang
dc.contributor.authorThomson, Benjamin R.
dc.contributor.authorToris, Carol B.
dc.contributor.authorWilloughby, Colin E.
dc.contributor.authorZhang, Hao F.
dc.contributor.authorFreddo, Thomas F.
dc.contributor.authorFuchshofer, Rudolf
dc.contributor.authorHill, Kamisha R.
dc.contributor.authorKarimi, Alireza
dc.contributor.authorKizhatil, Krishnakumar
dc.contributor.authorKopcyznski, Casey C.
dc.contributor.authorLiton, Paloma
dc.contributor.authorPatel, Gaurang
dc.contributor.authorPeng, Michael
dc.contributor.authorPattabiraman, Padmanabhan P.
dc.contributor.authorPrasanna, Ganesh
dc.contributor.authorReina-Torres, Ester
dc.contributor.authorSamples, E. Griffen
dc.contributor.authorSamples, John R.
dc.contributor.authorSteel, Cynthia L.
dc.contributor.authorStrohmaier, Clemens A.
dc.contributor.authorSubramanian, Preeti
dc.contributor.authorSugali, Chenna Kesavulu
dc.contributor.authorvan Batenburg-Sherwood, Joseph
dc.contributor.authorWong, Cydney
dc.contributor.authorYoungblood, Hannah
dc.contributor.authorZode, Gulab S.
dc.contributor.authorWhite, Elizabeth
dc.contributor.authorStamer, W. Daniel
dc.contributor.departmentOphthalmology, School of Medicine
dc.date.accessioned2025-02-24T16:44:27Z
dc.date.available2025-02-24T16:44:27Z
dc.date.issued2024
dc.description.abstractIntraocular pressure (IOP) elevation is the primary risk factor and currently the main treatable factor for progression of glaucomatous optic neuropathy. In addition to direct clinical and living animal in vivo studies, ex vivo perfusion of anterior segments and whole eyes is a key technique for studying conventional outflow function as it is responsible for IOP regulation. We present well-tested experimental details, protocols, considerations, advantages, and limitations of several ex vivo model systems for studying IOP regulation. These include: (1) perfused whole globes, (2) stationary anterior segment organ culture, (3) perfused human anterior segment organ culture, (4) perfused animal anterior segment organ culture, (5) perfused human corneal rims, and (6) perfused human anterior segment wedges. These methods, with due consideration paid to their strengths and limitations, comprise a set of very strong tools for extending our understanding of IOP regulation.
dc.eprint.versionFinal published version
dc.identifier.citationAcott TS, Fautsch MP, Mao W, et al. Consensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches. Invest Ophthalmol Vis Sci. 2024;65(14):32. doi:10.1167/iovs.65.14.32
dc.identifier.urihttps://hdl.handle.net/1805/45980
dc.language.isoen_US
dc.publisherAssociation for Research in Vision and Ophthalmology
dc.relation.isversionof10.1167/iovs.65.14.32
dc.relation.journalInvestigative Ophthalmology & Visual Science
dc.rightsAttribution-NonCommercial-NoDerivatives 4.0 Internationalen
dc.rights.urihttps://creativecommons.org/licenses/by-nc-nd/4.0
dc.sourcePMC
dc.subjectIntraocular pressure regulation models
dc.subjectPerfused anterior segment organ culture
dc.subjectEx vivo aqueous humor outflow regulation models
dc.titleConsensus Recommendations for Studies of Outflow Facility and Intraocular Pressure Regulation Using Ex Vivo Perfusion Approaches
dc.typeArticle
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