Molecular testing for gastrointestinal pathogens in intestinal tissue of infants with necrotizing enterocolitis or spontaneous intestinal perforation

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dc.contributor.authorTalavera-Barber, Maria M.
dc.contributor.authorSánchez, Pablo J.
dc.contributor.authorConces, Miriam
dc.contributor.authorKaptsan, Irina
dc.contributor.authorEverhart, Kathy
dc.contributor.authorLeber, Amy
dc.contributor.authorMalleske, Daniel T.
dc.contributor.authorMoallem, Mohannad
dc.contributor.authorPanesso-Gómez, Santiago
dc.contributor.authorShimamura, Masako
dc.contributor.departmentPediatrics, School of Medicine
dc.date.accessioned2025-01-28T10:19:07Z
dc.date.available2025-01-28T10:19:07Z
dc.date.issued2024
dc.description.abstractObjective: The objective of this study was to determine the frequency of common gastrointestinal bacterial, parasitic, and viral pathogen detection in necrotizing enterocolitis (NEC) or spontaneous intestinal perforation (SIP) -associated intestinal tissue. Study design: Retrospective cohort study examined formalin fixed, paraffin embedded (FFPE) surgical or autopsy intestinal tissue from NEC or SIP specimens. DNA and RNA were extracted and analyzed by multiplex PCR panel (GIFA Biofire). DNA or RNA from stool samples containing each pathogen were extracted for positive controls. Results: The total number of intestinal tissue samples were 193 from 310 infants (156 NEC, 37 SIP). Six (3%) infants with stage III NEC tested positive for a target pathogen; 2, C. difficile; 3, Enteroaggregtive E. coli; and 1, Giardia. No gastrointestinal viral pathogens were detected. Conclusion: Molecular testing yielded few GI pathogens suggesting that these organisms are likely not major causes or facilitators of NEC or SIP.
dc.eprint.versionFinal published version
dc.identifier.citationTalavera-Barber MM, Sánchez PJ, Conces M, et al. Molecular testing for gastrointestinal pathogens in intestinal tissue of infants with necrotizing enterocolitis or spontaneous intestinal perforation. J Perinatol. 2024;44(12):1755-1761. doi:10.1038/s41372-024-01999-z
dc.identifier.urihttps://hdl.handle.net/1805/45528
dc.language.isoen_US
dc.publisherSpringer Nature
dc.relation.isversionof10.1038/s41372-024-01999-z
dc.relation.journalJournal of Perinatology
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.sourcePMC
dc.subjectGastrointestinal diseases
dc.subjectDisease model
dc.subjectAcute inflammation
dc.titleMolecular testing for gastrointestinal pathogens in intestinal tissue of infants with necrotizing enterocolitis or spontaneous intestinal perforation
dc.typeArticle
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