LAMP-enabled diagnosis of Kaposi's sarcoma for sub-Saharan Africa

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2023
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American English
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American Association for the Advancement of Science
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Abstract

Kaposi's sarcoma (KS) is an endothelial cancer caused by the Kaposi's sarcoma-associated herpesvirus (KSHV) and is one of the most common cancers in sub-Saharan Africa. In limited-resource settings, traditional pathology infrastructure is often insufficient for timely diagnosis, leading to frequent diagnoses at advanced-stage disease where survival is poor. In this study, we investigate molecular diagnosis of KS performed in a point-of-care device to circumvent the limited infrastructure for traditional diagnosis. Using 506 mucocutaneous biopsies collected from patients at three HIV clinics in Uganda, we achieved 97% sensitivity, 92% specificity, and 96% accuracy compared to gold standard U.S.-based pathology. The results presented in this manuscript show that LAMP-based quantification of KSHV DNA extracted from KS-suspected biopsies has the potential to serve as a successful diagnostic for the disease and that diagnosis may be accurately achieved using a point-of-care device, reducing the barriers to obtaining KS diagnosis while increasing diagnostic accuracy.

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McCloskey D, Semeere A, Ayanga R, et al. LAMP-enabled diagnosis of Kaposi's sarcoma for sub-Saharan Africa. Sci Adv. 2023;9(2):eadc8913. doi:10.1126/sciadv.adc8913
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Science Advances
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PMC
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