Single-cell transcriptome and antigen-immunoglobin analysis reveals the diversity of B cells in non-small cell lung cancer
dc.contributor.author | Chen, Jian | |
dc.contributor.author | Tan, Yun | |
dc.contributor.author | Sun, Fenghuan | |
dc.contributor.author | Hou, Likun | |
dc.contributor.author | Zhang, Chi | |
dc.contributor.author | Ge, Tao | |
dc.contributor.author | Yu, Huansha | |
dc.contributor.author | Wu, Chunxiao | |
dc.contributor.author | Zhu, Yuming | |
dc.contributor.author | Duan, Liang | |
dc.contributor.author | Wu, Liang | |
dc.contributor.author | Song, Nan | |
dc.contributor.author | Zhang, Liping | |
dc.contributor.author | Zhang, Wei | |
dc.contributor.author | Wang, Di | |
dc.contributor.author | Chen, Chang | |
dc.contributor.author | Wu, Chunyan | |
dc.contributor.author | Jiang, Gening | |
dc.contributor.author | Zhang, Peng | |
dc.contributor.department | Medical and Molecular Genetics, School of Medicine | en_US |
dc.date.accessioned | 2020-11-09T16:13:39Z | |
dc.date.available | 2020-11-09T16:13:39Z | |
dc.date.issued | 2020-06-24 | |
dc.description.abstract | Background Malignant transformation and progression of cancer are driven by the co-evolution of cancer cells and their dysregulated tumor microenvironment (TME). Recent studies on immunotherapy demonstrate the efficacy in reverting the anti-tumoral function of T cells, highlighting the therapeutic potential in targeting certain cell types in TME. However, the functions of other immune cell types remain largely unexplored. Results We conduct a single-cell RNA-seq analysis of cells isolated from tumor tissue samples of non-small cell lung cancer (NSCLC) patients, and identify subtypes of tumor-infiltrated B cells and their diverse functions in the progression of NSCLC. Flow cytometry and immunohistochemistry experiments on two independent cohorts confirm the co-existence of the two major subtypes of B cells, namely the naïve-like and plasma-like B cells. The naïve-like B cells are decreased in advanced NSCLC, and their lower level is associated with poor prognosis. Co-culture of isolated naïve-like B cells from NSCLC patients with two lung cancer cell lines demonstrate that the naïve-like B cells suppress the growth of lung cancer cells by secreting four factors negatively regulating the cell growth. We also demonstrate that the plasma-like B cells inhibit cancer cell growth in the early stage of NSCLC, but promote cell growth in the advanced stage of NSCLC. The roles of the plasma-like B cell produced immunoglobulins, and their interacting proteins in the progression of NSCLC are further validated by proteomics data. Conclusion Our analysis reveals versatile functions of tumor-infiltrating B cells and their potential clinical implications in NSCLC. | en_US |
dc.identifier.citation | Chen, J., Tan, Y., Sun, F., Hou, L., Zhang, C., Ge, T., Yu, H., Wu, C., Zhu, Y., Duan, L., Wu, L., Song, N., Zhang, L., Zhang, W., Wang, D., Chen, C., Wu, C., Jiang, G., & Zhang, P. (2020). Single-cell transcriptome and antigen-immunoglobin analysis reveals the diversity of B cells in non-small cell lung cancer. Genome Biology, 21(1), 152. https://doi.org/10.1186/s13059-020-02064-6 | en_US |
dc.identifier.issn | 1474-760X | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/24329 | |
dc.language.iso | en_US | en_US |
dc.publisher | BMC | en_US |
dc.relation.isversionof | 10.1186/s13059-020-02064-6 | en_US |
dc.relation.journal | Genome Biology | en_US |
dc.rights | Attribution 4.0 International | * |
dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | * |
dc.source | PMC | en_US |
dc.subject | non-small cell lung cancer | en_US |
dc.subject | antigen-immunoglobin analysis | en_US |
dc.subject | Single-cell transcriptome | en_US |
dc.subject | B cells | en_US |
dc.title | Single-cell transcriptome and antigen-immunoglobin analysis reveals the diversity of B cells in non-small cell lung cancer | en_US |
dc.type | Article | en_US |
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