Dissolvable microgel-templated macroporous hydrogels for controlled cell assembly

dc.contributor.authorJiang, Zhongliang
dc.contributor.authorLin, Fang-Yi
dc.contributor.authorJiang, Kun
dc.contributor.authorNguyen, Han
dc.contributor.authorChang, Chun-Yi
dc.contributor.authorLin, Chien-Chi
dc.contributor.departmentBiomedical Engineering, School of Engineering and Technology
dc.date.accessioned2023-11-01T10:32:04Z
dc.date.available2023-11-01T10:32:04Z
dc.date.issued2022
dc.description.abstractMesenchymal stem cells (MSCs)-based therapies have been widely used to promote tissue regeneration and to modulate immune/inflammatory response. The therapeutic potential of MSCs can be further improved by forming multi-cellular spheroids. Meanwhile, hydrogels with macroporous structures are advantageous for improving mass transport properties for the cell-laden matrices. Herein, we report the fabrication of MSC-laden macroporous hydrogel scaffolds through incorporating rapidly dissolvable spherical cell-laden microgels. Dissolvable microgels were fabricated by tandem droplet-microfluidics and thiol-norbornene photopolymerization using a novel fast-degrading macromer poly(ethylene glycol)-norbornene-dopamine (PEGNB-Dopa). The cell-laden microgels were subsequently encapsulated within another bulk hydrogel matrix, whose porous structure was generated efficiently by the rapid degradation of the PEGNB-Dopa microgels. The cytocompatibility of this in situ pore-forming approach was demonstrated with multiple cell types. Furthermore, adjusting the stiffness and cell adhesiveness of the bulk hydrogels afforded the formation of solid cell spheroids or hollow spheres. The assembly of solid or hollow MSC spheroids led to differential activation of AKT pathway. Finally, MSCs solid spheroids formed in situ within the macroporous hydrogels exhibited robust secretion of HGF, VEGF-A, IL-6, IL-8, and TIMP-2. In summary, this platform provides an innovative method for forming cell-laden macroporous hydrogels for a variety of future biomedical applications.
dc.eprint.versionAuthor's manuscript
dc.identifier.citationJiang Z, Lin FY, Jiang K, Nguyen H, Chang CY, Lin CC. Dissolvable microgel-templated macroporous hydrogels for controlled cell assembly. Biomater Adv. 2022;134:112712. doi:10.1016/j.msec.2022.112712
dc.identifier.urihttps://hdl.handle.net/1805/36823
dc.language.isoen_US
dc.publisherElsevier
dc.relation.isversionof10.1016/j.msec.2022.112712
dc.relation.journalBiomaterials Advances
dc.rightsPublisher Policy
dc.sourcePMC
dc.subjectMacroporous hydrogel
dc.subjectDroplet-microfluidics
dc.subjectCell-laden microgels
dc.subjectMesenchymal stem cells
dc.titleDissolvable microgel-templated macroporous hydrogels for controlled cell assembly
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
nihms-1808141.pdf
Size:
2.61 MB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
1.99 KB
Format:
Item-specific license agreed upon to submission
Description: