Estrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal model

dc.contributor.authorPaschalis, E. P.
dc.contributor.authorGamsjaeger, S.
dc.contributor.authorCondon, Keith
dc.contributor.authorKlaushofer, K.
dc.contributor.authorBurr, David
dc.contributor.departmentAnatomy and Cell Biology, School of Medicineen_US
dc.date.accessioned2018-12-05T20:05:02Z
dc.date.available2018-12-05T20:05:02Z
dc.date.issued2019-03
dc.description.abstractOvariectomized animal models have been extensively used in osteoporosis research due to the resulting loss of bone mass. The purpose of the present study was to test the hypothesis that estrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal model. To achieve this we used Raman microspectroscopy to analyze humeri from monkeys that were either SHAM-operated or ovariectomized (N = 10 for each group). Measurements were made as a function of tissue age and cortical surface (periosteal, osteonal, endosteal) based on the presence of calcein fluorescent double labels. In the present work we focused on osteoid seams (defined as a surface with evident calcein labels, 1 μm distance away from the mineralizing front, and for which the Raman spectra showed the presence of organic matrix but not mineral), as well as the youngest mineralized tissue between the second fluorescent label and the mineralizing front, 1 μm inwards from the front with the phosphate mineral peak evident in the Raman spectra (TA1). The spectroscopically determined parameters of interest were the relative glycosaminoglycan (GAG) and pyridinoline (Pyd) contents in the osteoid, and the mineral content in TA1. At all three cortical surfaces, significant correlations were evident in the SHAM-operated animals between osteoid GAG (negative) and Pyd content, and mineral content, unlike the OVX animals. These results suggest that in addition to the well-established effects on turnover rates and bone mass, estrogen depletion alters the regulation of mineralization by GAGs and Pyd.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationPaschalis, E. P., Gamsjaeger, S., Condon, K., Klaushofer, K., & Burr, D. (2019). Estrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal model. Bone, 120, 279-284. https://doi.org/10.1016/j.bone.2018.11.004en_US
dc.identifier.urihttps://hdl.handle.net/1805/17907
dc.language.isoenen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.bone.2018.11.004en_US
dc.relation.journalBoneen_US
dc.rightsPublisher Policyen_US
dc.sourceAuthoren_US
dc.subjectestrogenen_US
dc.subjectglycosaminoglycansen_US
dc.subjectpyridinolineen_US
dc.titleEstrogen depletion alters mineralization regulation mechanisms in an ovariectomized monkey animal modelen_US
dc.typeArticleen_US
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