New Twists in Detecting mRNA Modification Dynamics

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Date
2020-07-01
Authors
Anreiter, Ina
Mir, Quoseena
Simpson, Jared T.
Janga, Sarath C.
Soller, Matthias
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American English
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BioHealth Informatics, School of Informatics and Computing
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Elsevier
Abstract

Modified nucleotides in mRNA are an essential addition to the standard genetic code of four nucleotides in animals, plants, and their viruses. The emerging field of epitranscriptomics examines nucleotide modifications in mRNA and their impact on gene expression. The low abundance of nucleotide modifications and technical limitations, however, have hampered systematic analysis of their occurrence and functions. Selective chemical and immunological identification of modified nucleotides has revealed global candidate topology maps for many modifications in mRNA, but further technical advances to increase confidence will be necessary. Single-molecule sequencing introduced by Oxford Nanopore now promises to overcome such limitations, and we summarize current progress with a particular focus on the bioinformatic challenges of this novel sequencing technology.

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This article is made available for unrestricted research re-use and secondary analysis in any form or be any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
Keywords
COVID-19, mRNA, mRNA Modifications, Epitranscriptomics, Nanopore
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Anreiter, I., Mir, Q., Simpson, J. T., Janga, S. C., & Soller, M. (2020). New Twists in Detecting mRNA Modification Dynamics. Trends in Biotechnology, 1–18. https://doi.org/10.1016/j.tibtech.2020.06.002
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0167-7799
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For this work, we acknowledge funding from the UK Biotechnology and Biological Sciences Research Council (BBSRC) and the Leverhulme trust to M.S. I.A. is supported by the Schmidt Science Fellows in partnership with the Rhodes Trust. J.T.S. is supported by the Ontario Institute for Cancer Research through funds provided by the Government of Ontario and theGovernment of Canada via Genome Canada and Ontario Genomics (OGI-136). S.C.J. acknowledges support from the National Institute of General Medical Sciences of the National Institutes of Health (grant R01GM123314) and the National Science Foundation (1940422 and 1908992).
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Trends in Biotechnology
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Public Health Emergency
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https://hdl.handle.net/1805/23279
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https://doi.org/10.1016/j.tibtech.2020.06.002
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Final published version
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