The Effect of Single Versus Group μCT on the Detection of Trabecular and Cortical Disease Phenotypes in Mouse Bones

dc.contributor.authorKohler, Rachel
dc.contributor.authorTastad, Carli A.
dc.contributor.authorStacy, Alexander J.
dc.contributor.authorSwallow, Elizabeth A.
dc.contributor.authorMetzger, Corinne E.
dc.contributor.authorAllen, Matthew R.
dc.contributor.authorWallace, Joseph M.
dc.contributor.departmentBiomedical Engineering, School of Engineering and Technologyen_US
dc.date.accessioned2022-08-01T16:46:29Z
dc.date.available2022-08-01T16:46:29Z
dc.date.issued2021-03-05
dc.description.abstractMicro‐computed tomography is a critical assessment tool for bone‐related preclinical research, especially in murine models. To expedite the scanning process, researchers often image multiple bones simultaneously; however, it is unknown if this impacts scan quality and alters the ability to detect differences between experimental groups. The purpose of this study was to assess the effect of multibone scanning on detecting disease‐induced changes in bone microarchitecture and mineral density by group scanning two murine models with known skeletal defects: the Col1a2 G610C/+ model of osteogenesis imperfecta and an adenine‐induced model of chronic kidney disease. Adult male femurs were scanned individually and in random groups of three and eight in a Bruker Skyscan 1172 and 1176, respectively, then assessed for standard trabecular and cortical bone measures. Although scanning methodology altered raw values, with trabecular microarchitecture values more affected than cortical properties, a disease phenotype was still detectable in both group and solo scans. However, tissue mineral density in both trabecular and cortical bone was significantly impacted by group versus solo scanning. Researchers may be able to use small groupings in a single μCT scan to expedite preclinical analyses when the overall bone phenotype is large to decrease costs and increase speed of discoveries; however the details of scanning (single or group) should always be reported.en_US
dc.eprint.versionFinal published versionen_US
dc.identifier.citationKohler R, Tastad CA, Stacy AJ, et al. The Effect of Single Versus Group μCT on the Detection of Trabecular and Cortical Disease Phenotypes in Mouse Bones. JBMR Plus. 2021;5(4):e10473. Published 2021 Mar 5. doi:10.1002/jbm4.10473en_US
dc.identifier.urihttps://hdl.handle.net/1805/29686
dc.language.isoen_USen_US
dc.publisherWileyen_US
dc.relation.isversionof10.1002/jbm4.10473en_US
dc.relation.journalJBMR Plusen_US
dc.rightsAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.sourcePMCen_US
dc.subjectBone micro-computed tomographyen_US
dc.subjectChronic kidney diseaseen_US
dc.subjectOsteogenesis imperfectaen_US
dc.titleThe Effect of Single Versus Group μCT on the Detection of Trabecular and Cortical Disease Phenotypes in Mouse Bonesen_US
dc.typeArticleen_US
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