Characterization of a novel polyclonal anti-hypusine antibody
| dc.contributor.author | Nishiki, Yurika | |
| dc.contributor.author | Farb, Thomas B. | |
| dc.contributor.author | Friedrich, Jessica | |
| dc.contributor.author | Bokvist, Krister | |
| dc.contributor.author | Mirmira, Raghavendra G. | |
| dc.contributor.author | Maier, Bernhard | |
| dc.contributor.department | Pediatrics, School of Medicine | |
| dc.date.accessioned | 2025-05-01T11:17:06Z | |
| dc.date.available | 2025-05-01T11:17:06Z | |
| dc.date.issued | 2013-08-29 | |
| dc.description.abstract | The translation factor eIF5A is the only protein known to contain the amino acid hypusine, which is formed posttranslationally. Hypusinated eIF5A is necessary for cellular proliferation and responses to extracellular stressors, and has been proposed as a target for pharmacologic therapy. Here, we provide the first comprehensive characterization of a novel polyclonal antibody (IU-88) that specifically recognizes the hypusinated eIF5A. IU-88 will be useful for the investigation of eIF5A biology and for the development of assays recognizing hypusinated eIF5A. | |
| dc.eprint.version | Final published version | |
| dc.identifier.citation | Nishiki Y, Farb TB, Friedrich J, Bokvist K, Mirmira RG, Maier B. Characterization of a novel polyclonal anti-hypusine antibody. Springerplus. 2013;2:421. Published 2013 Aug 29. doi:10.1186/2193-1801-2-421 | |
| dc.identifier.uri | https://hdl.handle.net/1805/47584 | |
| dc.language.iso | en_US | |
| dc.publisher | Springer | |
| dc.relation.isversionof | 10.1186/2193-1801-2-421 | |
| dc.relation.journal | SpringerPlus | |
| dc.rights | Attribution 4.0 International | en |
| dc.rights.uri | http://creativecommons.org/licenses/by/4.0/ | |
| dc.source | PMC | |
| dc.subject | Hypusine | |
| dc.subject | eIF5A | |
| dc.subject | Antibody | |
| dc.subject | Recombinant proteins | |
| dc.subject | Cell lines | |
| dc.title | Characterization of a novel polyclonal anti-hypusine antibody | |
| dc.type | Article |