Global microRNA expression is essential for murine mast cell development in vivo
dc.contributor.author | Oh, Sun Young | |
dc.contributor.author | Brandal, Stephanie | |
dc.contributor.author | Kapur, Reuben | |
dc.contributor.author | Zhu, Zhou | |
dc.contributor.author | Takemoto, Clifford | |
dc.contributor.department | Department of Pediatrics, IU School of Medicine | en_US |
dc.date.accessioned | 2016-08-19T19:17:42Z | |
dc.date.available | 2016-08-19T19:17:42Z | |
dc.date.issued | 2014-10 | |
dc.description.abstract | MicroRNAs (miRNAs) are small, noncoding RNAs that have been shown to play a critical role in normal physiology and disease, such as hematopoietic development and cancer. However, their role in mast-cell function and development is poorly understood. The major objective of this study was to determine how global miRNA expression affects mast-cell physiology. The RNase III endonuclease, Dicer, is required for the processing of pre-miRNAs into mature miRNAs. To investigate the effect of global miRNA depletion on mast cells in vivo, we generated a mast-cell-specific knock out of Dicer in mice. Transgenic mice (Mcpt5-Cre) that express Cre selectively in connective tissue mast cells were crossed with mice carrying the floxed conditional Dicer allele (Dicer fl/fl). Mcpt5-Cre × Dicer fl/fl mice with homozygous Dicer gene deletion in mast cells were found to have a profound mast-cell deficiency with near complete loss of peritoneal, gastrointestinal, and skin mast cells. We examined the in vivo functional consequence of mast-cell-specific Dicer deletion using an immunoglobulin-E-dependent passive systemic anaphylaxis murine model. Immunoglobulin-E-sensitized wild type Mcpt5-Cre × Dicer +/+ and heterozygous Mcpt5-Cre × Dicer fl/+ mice show marked hypothermia with antigen; however, homozygous Mcpt5-Cre × Dicer fl/fl mice were completely unresponsive to antigen challenge. These studies suggest a critical role for Dicer and miRNA expression for establishment of tissue compartments of functional mast cells in vivo | en_US |
dc.eprint.version | Author's manuscript | en_US |
dc.identifier.citation | Oh, S. Y., Brandal, S., Kapur, R., Zhu, Z., & Takemoto, C. M. (2014). Global microRNA expression is essential for murine mast cell development in vivo. Experimental Hematology, 42(10), 919–923.e1. http://doi.org/10.1016/j.exphem.2014.07.266 | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/10740 | |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | 10.1016/j.exphem.2014.07.266 | en_US |
dc.relation.journal | Experimental Hematology | en_US |
dc.rights | Publisher Policy | en_US |
dc.source | PMC | en_US |
dc.subject | DEAD-box RNA Helicases | en_US |
dc.subject | Mast Cells | en_US |
dc.subject | Ovalbumin | en_US |
dc.subject | Peritoneum | en_US |
dc.subject | Ribonuclease III | en_US |
dc.subject | Skin | en_US |
dc.subject | Stomach | en_US |
dc.title | Global microRNA expression is essential for murine mast cell development in vivo | en_US |
dc.type | Article | en_US |