Protocol for enrichment and functional analysis of human hematopoietic cells from umbilical cord blood

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dc.contributor.authorGutch, Sarah
dc.contributor.authorBeasley, Lindsay
dc.contributor.authorCooper, Scott
dc.contributor.authorKaplan, Mark H.
dc.contributor.authorCapitano, Maegan L.
dc.contributor.authorRopa, James
dc.contributor.departmentMicrobiology and Immunology, School of Medicine
dc.date.accessioned2024-08-01T12:42:00Z
dc.date.available2024-08-01T12:42:00Z
dc.date.issued2024
dc.description.abstractUmbilical cord blood (CB) is a donor source for hematopoietic cell therapies. Understanding what drives hematopoietic stem and progenitor cell function is critical to our understanding of the usage of CB in hematopoietic cell therapies. Here, we describe how to isolate and analyze the function of human hematopoietic cells from umbilical CB. This protocol demonstrates assays that measure phenotypic properties and hematopoietic cell potency. For complete details on the use and execution of this protocol, please refer to Broxmeyer et al.
dc.eprint.versionFinal published version
dc.identifier.citationGutch S, Beasley L, Cooper S, Kaplan MH, Capitano ML, Ropa J. Protocol for enrichment and functional analysis of human hematopoietic cells from umbilical cord blood. STAR Protoc. 2024;5(2):103024. doi:10.1016/j.xpro.2024.103024
dc.identifier.urihttps://hdl.handle.net/1805/42531
dc.language.isoen_US
dc.publisherElsevier
dc.relation.isversionof10.1016/j.xpro.2024.103024
dc.relation.journalSTAR Protocols
dc.rightsAttribution-NonCommercial 4.0 Internationalen
dc.rights.urihttps://creativecommons.org/licenses/by-nc/4.0
dc.sourcePMC
dc.subjectCell differentiation
dc.subjectCell culture
dc.subjectCell isolation
dc.subjectStem cells
dc.titleProtocol for enrichment and functional analysis of human hematopoietic cells from umbilical cord blood
dc.typeArticle
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