Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell Function

dc.contributor.authorPankajakshan, Divya
dc.contributor.authorAlbuquerque, Maria T.P.
dc.contributor.authorEvans, Joshua D.
dc.contributor.authorKamocka, Malgorzata M.
dc.contributor.authorGregory, Richard L.
dc.contributor.authorBottino, Marco C.
dc.contributor.departmentBiomedical and Applied Sciences, School of Dentistryen_US
dc.date.accessioned2018-03-14T18:41:05Z
dc.date.available2018-03-14T18:41:05Z
dc.date.issued2016-10
dc.description.abstractIntroduction Root canal disinfection and the establishment of an intracanal microenvironment conducive to the proliferation/differentiation of stem cells play a significant role in regenerative endodontics. This study was designed to (1) investigate the antimicrobial efficacy of triple antibiotic–containing nanofibers against a dual-species biofilm and (2) evaluate the ability of dental pulp stem cells (DPSCs) to adhere to and proliferate on dentin upon nanofiber exposure. Methods Seven-day-old dual-species biofilm established on dentin specimens was exposed for 3 days to the following: saline (control), antibiotic-free nanofibers (control), and triple antibiotic–containing nanofibers or a saturated triple antibiotic paste (TAP) solution (50 mg/mL in phosphate buffer solution). Bacterial viability was assessed using the LIVE/DEAD assay (Molecular Probes, Inc, Eugene, OR) and confocal laser scanning microscopy. For cyto-compatibility studies, dentin specimens after nanofiber or TAP (1 g/mL in phosphate buffer solution) exposure were evaluated for cell adhesion and spreading by actin-phalloidin staining. DPSC proliferation was assessed on days 1, 3, and 7. Statistics were performed, and significance was set at the 5% level. Results Confocal laser scanning microscopy showed significant bacterial death upon antibiotic-containing nanofiber exposure, differing significantly (P < .05) from antibiotic-free fibers and the control (saline). DPSCs showed enhanced adhesion/spreading on dentin specimens treated with antibiotic-containing nanofibers when compared with its TAP counterparts. The DPSC proliferation rate was similar on days 1 and 3 in antibiotic-free nanofibers, triple antibiotic–containing nanofibers, and TAP-treated dentin. Proliferation was higher (9-fold) on dentin treated with antibiotic-containing nanofibers on day 7 compared with TAP. Conclusions Triple antibiotic–containing polymer nanofibers led to significant bacterial death, whereas they did not affect DPSC attachment and proliferation on dentin.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationPankajakshan, D., Albuquerque, M. T. P., Evans, J. D., Kamocka, M. M., Gregory, R. L., & Bottino, M. C. (2016). Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell Function. Journal of Endodontics, 42(10), 1490–1495. https://doi.org/10.1016/j.joen.2016.07.019en_US
dc.identifier.issn0099-2399en_US
dc.identifier.urihttps://hdl.handle.net/1805/15542
dc.language.isoen_USen_US
dc.publisherElsevieren_US
dc.relation.isversionof10.1016/j.joen.2016.07.019en_US
dc.relation.journalJournal of endodonticsen_US
dc.rightsPublisher Policyen_US
dc.sourcePMCen_US
dc.subjectAntibioticen_US
dc.subjectdisinfectionen_US
dc.subjectelectrospinningen_US
dc.subjectnanofibersen_US
dc.subjectregenerationen_US
dc.subjectstem cellsen_US
dc.titleTriple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell Functionen_US
dc.typeArticleen_US
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