Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell Function
dc.contributor.author | Pankajakshan, Divya | |
dc.contributor.author | Albuquerque, Maria T.P. | |
dc.contributor.author | Evans, Joshua D. | |
dc.contributor.author | Kamocka, Malgorzata M. | |
dc.contributor.author | Gregory, Richard L. | |
dc.contributor.author | Bottino, Marco C. | |
dc.contributor.department | Biomedical and Applied Sciences, School of Dentistry | en_US |
dc.date.accessioned | 2018-03-14T18:41:05Z | |
dc.date.available | 2018-03-14T18:41:05Z | |
dc.date.issued | 2016-10 | |
dc.description.abstract | Introduction Root canal disinfection and the establishment of an intracanal microenvironment conducive to the proliferation/differentiation of stem cells play a significant role in regenerative endodontics. This study was designed to (1) investigate the antimicrobial efficacy of triple antibiotic–containing nanofibers against a dual-species biofilm and (2) evaluate the ability of dental pulp stem cells (DPSCs) to adhere to and proliferate on dentin upon nanofiber exposure. Methods Seven-day-old dual-species biofilm established on dentin specimens was exposed for 3 days to the following: saline (control), antibiotic-free nanofibers (control), and triple antibiotic–containing nanofibers or a saturated triple antibiotic paste (TAP) solution (50 mg/mL in phosphate buffer solution). Bacterial viability was assessed using the LIVE/DEAD assay (Molecular Probes, Inc, Eugene, OR) and confocal laser scanning microscopy. For cyto-compatibility studies, dentin specimens after nanofiber or TAP (1 g/mL in phosphate buffer solution) exposure were evaluated for cell adhesion and spreading by actin-phalloidin staining. DPSC proliferation was assessed on days 1, 3, and 7. Statistics were performed, and significance was set at the 5% level. Results Confocal laser scanning microscopy showed significant bacterial death upon antibiotic-containing nanofiber exposure, differing significantly (P < .05) from antibiotic-free fibers and the control (saline). DPSCs showed enhanced adhesion/spreading on dentin specimens treated with antibiotic-containing nanofibers when compared with its TAP counterparts. The DPSC proliferation rate was similar on days 1 and 3 in antibiotic-free nanofibers, triple antibiotic–containing nanofibers, and TAP-treated dentin. Proliferation was higher (9-fold) on dentin treated with antibiotic-containing nanofibers on day 7 compared with TAP. Conclusions Triple antibiotic–containing polymer nanofibers led to significant bacterial death, whereas they did not affect DPSC attachment and proliferation on dentin. | en_US |
dc.eprint.version | Author's manuscript | en_US |
dc.identifier.citation | Pankajakshan, D., Albuquerque, M. T. P., Evans, J. D., Kamocka, M. M., Gregory, R. L., & Bottino, M. C. (2016). Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell Function. Journal of Endodontics, 42(10), 1490–1495. https://doi.org/10.1016/j.joen.2016.07.019 | en_US |
dc.identifier.issn | 0099-2399 | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/15542 | |
dc.language.iso | en_US | en_US |
dc.publisher | Elsevier | en_US |
dc.relation.isversionof | 10.1016/j.joen.2016.07.019 | en_US |
dc.relation.journal | Journal of endodontics | en_US |
dc.rights | Publisher Policy | en_US |
dc.source | PMC | en_US |
dc.subject | Antibiotic | en_US |
dc.subject | disinfection | en_US |
dc.subject | electrospinning | en_US |
dc.subject | nanofibers | en_US |
dc.subject | regeneration | en_US |
dc.subject | stem cells | en_US |
dc.title | Triple Antibiotic Polymer Nanofibers for Intracanal Drug Delivery: Effects on Dual Species Biofilm and Cell Function | en_US |
dc.type | Article | en_US |