MiR-24 Promotes the Survival of Hematopoietic Cells

dc.contributor.authorNguyen, Tan
dc.contributor.authorRich, Audrey
dc.contributor.authorDahl, Richard
dc.contributor.departmentMicrobiology and Immunology, School of Medicine
dc.date.accessioned2025-05-27T09:53:39Z
dc.date.available2025-05-27T09:53:39Z
dc.date.issued2013
dc.description.abstractThe microRNA, miR-24, inhibits B cell development and promotes myeloid development of hematopoietic progenitors. Differential regulation of cell survival in myeloid and lymphoid cells by miR-24 may explain how miR-24's affects hematopoietic progenitors. MiR-24 is reported to regulate apoptosis, either positively or negatively depending on cell context. However, no role for miR-24 in regulating cell death has been previously described in blood cells. To examine miR-24's effect on survival, we expressed miR-24 via retrovirus in hematopoietic cells and induced cell death with cytokine or serum withdrawal. We observed that miR-24 enhanced survival of myeloid and B cell lines as well as primary hematopoietic cells. Additionally, antagonizing miR-24 with shRNA in hematopoietic cells made them more sensitive to apoptotic stimuli, suggesting miR-24 functions normally to promote blood cell survival. Since we did not observe preferential protection of myeloid over B cells, miR-24's pro-survival effect does not explain its promotion of myelopoiesis. Moreover, expression of pro-survival protein, Bcl-xL, did not mimic miR-24's impact on cellular differentiation, further supporting this conclusion. Our results indicate that miR-24 is a critical regulator of hematopoietic cell survival. This observation has implications for leukemogenesis. Several miRNAs that regulate apoptosis have been shown to function as either tumor suppressors or oncogenes during leukemogenesis. MiR-24 is expressed highly in primary acute myelogenous leukemia, suggesting that its pro-survival activity could contribute to the transformation of hematopoietic cells.
dc.eprint.versionFinal published version
dc.identifier.citationNguyen T, Rich A, Dahl R. MiR-24 promotes the survival of hematopoietic cells. PLoS One. 2013;8(1):e55406. doi:10.1371/journal.pone.0055406
dc.identifier.urihttps://hdl.handle.net/1805/48367
dc.language.isoen_US
dc.publisherPublic Library of Science
dc.relation.isversionof10.1371/journal.pone.0055406
dc.relation.journalPLoS One
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourcePMC
dc.subjectApoptosis
dc.subjectHematopoietic stem cells
dc.subjectCell survival
dc.subjectImmunoblotting
dc.subjectRetroviridae
dc.titleMiR-24 Promotes the Survival of Hematopoietic Cells
dc.typeArticle
Files
Original bundle
Now showing 1 - 1 of 1
Loading...
Thumbnail Image
Name:
Nguyen2013Promotes-CCBY.pdf
Size:
894.63 KB
Format:
Adobe Portable Document Format
License bundle
Now showing 1 - 1 of 1
No Thumbnail Available
Name:
license.txt
Size:
2.04 KB
Format:
Item-specific license agreed upon to submission
Description: