Dihydroceramides Derived from Bacteroidetes Species Sensitize TRPV1 Channels

dc.contributor.authorLudwig, Nora
dc.contributor.authorDemaree, Isaac S.
dc.contributor.authorYamada, Chiaki
dc.contributor.authorNusbaum, Amilia
dc.contributor.authorNichols, Frank C.
dc.contributor.authorWhite, Fletcher A.
dc.contributor.authorMovila, Alexandru
dc.contributor.authorObukhov, Alexander G.
dc.contributor.departmentAnatomy, Cell Biology and Physiology, School of Medicine
dc.date.accessioned2023-10-18T12:37:57Z
dc.date.available2023-10-18T12:37:57Z
dc.date.issued2023-01-03
dc.description.abstractBacterial colonization of open wounds is common, and patients with infected wounds often report significantly elevated pain sensitivity at the wound site. Transient Receptor Potential Vanilloid Type 1 (TRPV1) channels are known to play an important role in pain signaling and may be sensitized under pro-inflammatory conditions. Bacterial membrane components, such as phosphoethanolamine dihydroceramide (PEDHC), phosphoglycerol dihydroceramide (PGDHC), and lipopolysaccharide (LPS), are released in the environment from the Gram-negative bacteria of the Bacteroidetes species colonizing the infected wounds. Here, we used intracellular calcium imaging and patch-clamp electrophysiology approaches to determine whether bacterially derived PEDHC, PGDHC, or LPS can modulate the activity of the TRPV1 channels heterologously expressed in HEK cells. We found that PEDHC and PGDHC can sensitize TRPV1 in a concentration-dependent manner, whereas LPS treatment does not significantly affect TRPV1 activity in HEK cells. We propose that sensitization of TRPV1 channels by Bacteroidetes-derived dihydroceramides may at least in part underlie the increased pain sensitivity associated with wound infections.
dc.eprint.versionFinal published version
dc.identifier.citationLudwig N, Demaree IS, Yamada C, et al. Dihydroceramides Derived from Bacteroidetes Species Sensitize TRPV1 Channels. Int J Mol Sci. 2023;24(1):877. Published 2023 Jan 3. doi:10.3390/ijms24010877
dc.identifier.urihttps://hdl.handle.net/1805/36430
dc.language.isoen_US
dc.publisherMDPI
dc.relation.isversionof10.3390/ijms24010877
dc.relation.journalInternational Journal of Molecular Sciences
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.sourcePMC
dc.subjectDihydroceramides
dc.subjectBacteroidetes
dc.subjectLipopolysaccharides
dc.subjectCapsaicin
dc.subjectPain
dc.titleDihydroceramides Derived from Bacteroidetes Species Sensitize TRPV1 Channels
dc.typeArticle
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