Norbornene-functionalized methylcellulose as a thermo- and photo-responsive bioink

dc.contributor.authorKim, Min Hee
dc.contributor.authorLin, Chien-Chi
dc.contributor.departmentBiomedical Engineering, School of Engineering and Technology
dc.date.accessioned2023-08-29T10:29:47Z
dc.date.available2023-08-29T10:29:47Z
dc.date.issued2021-09-21
dc.description.abstract3D bioprinting has emerged as an important tool to fabricate scaffolds with complex structures for tissue engineering and regenerative medicine applications. For extrusion-based 3D bioprinting, the success of printing complex structures relies largely on the properties of bioink. Methylcellulose (MC) has been exploited as a potential bioink for 3D bioprinting due to its temperature-dependent rheological properties. However, MC is highly soluble and has low structural stability at room temperature, making it suboptimal for 3D bioprinting applications. In this study, we report a one-step synthesis protocol for modifying MC with norbornene (MCNB), which serves as a new bioink for 3D bioprinting. MCNB preserves the temperature-dependent reversible sol-gel transition and readily reacts with thiol-bearing linkers through light-mediated step-growth thiol-norbornene photopolymerization. Furthermore, we rendered the otherwise inert MC network bioactive through facile conjugation of integrin-binding ligands (e.g., CRGDS) or via incorporating cell-adhesive and protease-sensitive gelatin-based macromer (e.g., GelNB). The adaptability of the new MCNB-based bioink offers an attractive option for diverse 3D bioprinting applications.
dc.eprint.versionAuthor's manuscript
dc.identifier.citationKim MH, Lin CC. Norbornene-functionalized methylcellulose as a thermo- and photo-responsive bioink. Biofabrication. 2021;13(4):10.1088/1758-5090/ac24dc. Published 2021 Sep 21. doi:10.1088/1758-5090/ac24dc
dc.identifier.urihttps://hdl.handle.net/1805/35200
dc.language.isoen_US
dc.publisherIOP
dc.relation.isversionof10.1088/1758-5090/ac24dc
dc.relation.journalBiofabrication
dc.rightsPublisher Policy
dc.sourcePMC
dc.subjectMethylcellulose
dc.subjectBioink
dc.subjectThiol-norbornene hydrogel
dc.subjectThermo-responsive
dc.subjectBioprinting
dc.titleNorbornene-functionalized methylcellulose as a thermo- and photo-responsive bioink
dc.typeArticle
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