Circulating Tumor Cells In Advanced Cervical Cancer: NRG Oncology-Gynecologic Oncology Group Study 240 (NCT 00803062)

dc.contributor.authorTewari, Krishnansu S.
dc.contributor.authorSill, Michael W.
dc.contributor.authorMonk, Bradley J.
dc.contributor.authorPenson, Richard T.
dc.contributor.authorMoore, David H.
dc.contributor.authorLankes, Heather A.
dc.contributor.authorRamondetta, Lois M.
dc.contributor.authorLandrum, Lisa M.
dc.contributor.authorRandall, Leslie M.
dc.contributor.authorOaknin, Ana
dc.contributor.authorLeitao, Mario M.
dc.contributor.authorEisenhauer, Eric L.
dc.contributor.authorDiSilvestro, Paul
dc.contributor.authorLe, Linda Van
dc.contributor.authorPearl, Michael L.
dc.contributor.authorBurke, James J.
dc.contributor.authorSalani, Ritu
dc.contributor.authorRichardson, Debra L.
dc.contributor.authorMichael, Helen E.
dc.contributor.authorKindelberger, David W.
dc.contributor.authorBirrer, Michael J.
dc.contributor.departmentPathology and Laboratory Medicine, School of Medicineen_US
dc.date.accessioned2023-03-03T15:05:34Z
dc.date.available2023-03-03T15:05:34Z
dc.date.issued2020-11
dc.description.abstractTo isolate circulating tumor cells (CTCs) from women with advanced cervical cancer and estimate the impact of CTCs and treatment on overall survival (OS) and progression-free survival (PFS). 7.5 mL of whole blood was drawn pre-cycle 1 and 36 days post-cycle 1 from patients enrolled on Gynecologic Oncology Group 0240, the phase III randomized trial that led directly to regulatory approval of the anti-angiogenesis drug, bevacizumab, in women with recurrent/metastatic cervical cancer. CTCs (defined as anti-cytokeratin positive/anti-CD45 negative cells) were isolated from the buffy coat layer using an anti-EpCAM antibody-conjugated ferrofluid and rare earth magnet, and counted using a semi-automated fluorescence microscope. The median pre-cycle 1 CTC count was 7 CTCs/7.5 mL whole blood (range, 0–18) and, at 36 days post-treatment, was 4 (range, 0–17). The greater the declination in CTCs between time points studied, the lower the risk of death (HR 0.87; 95% CI, 0.79–0.95). Among patients with high (≥ median) pre-treatment CTCs, bevacizumab treatment was associated with a reduction in the hazard of death (HR 0.57; 95% CI, 0.32–1.03) and progression (PFS HR 0.59; 95% CI, 0.36–0.96). This effect was not observed with low (< median) CTCs. CTCs can be isolated from women with advanced cervical cancer and may have prognostic significance. A survival benefit conferred by bevacizumab among patients with high pre-treatment CTCs may reflect increased tumor neovascularization and concomitant vulnerability to VEGF inhibition. These data support studying CTC capture as a potential predictive biomarker.en_US
dc.eprint.versionAuthor's manuscripten_US
dc.identifier.citationTewari KS, Sill MW, Monk BJ, et al. Circulating Tumor Cells In Advanced Cervical Cancer: NRG Oncology-Gynecologic Oncology Group Study 240 (NCT 00803062). Mol Cancer Ther. 2020;19(11):2363-2370. doi:10.1158/1535-7163.MCT-20-0276en_US
dc.identifier.urihttps://hdl.handle.net/1805/31593
dc.language.isoen_USen_US
dc.publisherAmerican Association for Cancer Researchen_US
dc.relation.isversionof10.1158/1535-7163.MCT-20-0276en_US
dc.relation.journalMolecular Cancer Therapeuticsen_US
dc.rightsPublisher Policyen_US
dc.sourcePMCen_US
dc.subjectCervical canceren_US
dc.subjectCirculating tumor cellsen_US
dc.subjectAngiogenesisen_US
dc.subjectBevacizumaben_US
dc.titleCirculating Tumor Cells In Advanced Cervical Cancer: NRG Oncology-Gynecologic Oncology Group Study 240 (NCT 00803062)en_US
dc.typeArticleen_US
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