Differential effects of brefeldin A on hormonally regulated Na + transport in a model renal epithelial cell line
dc.contributor.author | Blazer-Yost, Bonnie | |
dc.contributor.author | Coupaye-Gerard, Brigitte | |
dc.contributor.author | Kim, Hyun Jin | |
dc.contributor.author | Singh, Anup | |
dc.date.accessioned | 2018-07-19T18:09:40Z | |
dc.date.available | 2018-07-19T18:09:40Z | |
dc.date.issued | 1994 | |
dc.description.abstract | Na+ transport in renal epithelia is regulated by a wide variety of endogenous and exogenous cellular factors. Although most natriferic agents have an action on the amiloride-sensitive Na+ channel, the biochemical pathways which precede activation of the channel remain incompletely defined. One approach to dissecting such intricate pathways is to perturb a specific cellular process and determine its importance in the postulated mechanism. The current studies examine the effect of brefeldin A (BFA), an inhibitor of the central vacuolar system, on basal as well as aldosterone-, insulin-, and forskolin-stimulated Na+ transport. In the A6 cell line, BFA had a time-dependent effect on basal transport. Aldosterone-induced Na+ transport was sensitive to BFA while insulin's action was only partially blocked and forskolin-stimulated Na+ transport was relatively resistant to the action of the inhibitor. These studies highlight differences as well as points of convergence in the natriferic pathways. | en_US |
dc.identifier.citation | Brigitte Coupaye-Gerard, Hyun Jin Kim, Anup Singh, and Bonnie L. Blazer-Yost. Differential effects of brefeldin A on hormonally regulated Na + transport in a model renal epithelial cell line. Biochimica et Biophysica Acta. (1994). | en_US |
dc.identifier.uri | https://hdl.handle.net/1805/16719 | |
dc.subject | Cyclopentanes | en_US |
dc.subject | Hormones | en_US |
dc.subject | Kidney | en_US |
dc.subject | Sodium | en_US |
dc.title | Differential effects of brefeldin A on hormonally regulated Na + transport in a model renal epithelial cell line | en_US |
dc.type | Article | en_US |