A method to isolate the CTD of RNA Polymerase II for proteomics analysis

dc.contributor.advisorMosley, Amber L.
dc.contributor.authorAlakhras, Nada S.
dc.contributor.otherGoebl, Mark G.
dc.contributor.otherHurley, Thomas D., 1961-
dc.date.accessioned2015-04-14T14:24:54Z
dc.date.available2015-12-02T10:30:34Z
dc.date.issued2014-12
dc.degree.date2014en_US
dc.degree.disciplineDepartment of Biochemistry & Molecular Biologyen
dc.degree.grantorIndiana Universityen_US
dc.degree.levelM.S.en_US
dc.descriptionIndiana University-Purdue University Indianapolis (IUPUI)en_US
dc.description.abstractIn an effort to advance the methodology in analyzing RNAPII protein-protein interaction network and to determine the role of the CTD in controlling RNAPII transcription, we devised a method to specifically isolate the CTD-associated and CTD-less RNAPII to identify the proteins that interact with both the CTD and the globular core of RNAPII using novel purification scheme coupled to quantitative proteomics.en_US
dc.identifier.urihttps://hdl.handle.net/1805/6179
dc.identifier.urihttp://dx.doi.org/10.7912/C2/1882
dc.language.isoen_USen_US
dc.subject.lcshRNA polymerasesen_US
dc.subject.lcshRNA-protein interactionsen_US
dc.subject.lcshProtein-protein interactionsen_US
dc.subject.lcshRetrovirusesen_US
dc.subject.lcshQualitative researchen_US
dc.titleA method to isolate the CTD of RNA Polymerase II for proteomics analysisen_US
dc.typeThesisen
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