A method to isolate the CTD of RNA Polymerase II for proteomics analysis
| dc.contributor.advisor | Mosley, Amber L. | |
| dc.contributor.author | Alakhras, Nada S. | |
| dc.contributor.other | Goebl, Mark G. | |
| dc.contributor.other | Hurley, Thomas D., 1961- | |
| dc.date.accessioned | 2015-04-14T14:24:54Z | |
| dc.date.available | 2015-12-02T10:30:34Z | |
| dc.date.issued | 2014-12 | |
| dc.degree.date | 2014 | en_US |
| dc.degree.discipline | Department of Biochemistry & Molecular Biology | en |
| dc.degree.grantor | Indiana University | en_US |
| dc.degree.level | M.S. | en_US |
| dc.description | Indiana University-Purdue University Indianapolis (IUPUI) | en_US |
| dc.description.abstract | In an effort to advance the methodology in analyzing RNAPII protein-protein interaction network and to determine the role of the CTD in controlling RNAPII transcription, we devised a method to specifically isolate the CTD-associated and CTD-less RNAPII to identify the proteins that interact with both the CTD and the globular core of RNAPII using novel purification scheme coupled to quantitative proteomics. | en_US |
| dc.identifier.uri | https://hdl.handle.net/1805/6179 | |
| dc.identifier.uri | http://dx.doi.org/10.7912/C2/1882 | |
| dc.language.iso | en_US | en_US |
| dc.subject.lcsh | RNA polymerases | en_US |
| dc.subject.lcsh | RNA-protein interactions | en_US |
| dc.subject.lcsh | Protein-protein interactions | en_US |
| dc.subject.lcsh | Retroviruses | en_US |
| dc.subject.lcsh | Qualitative research | en_US |
| dc.title | A method to isolate the CTD of RNA Polymerase II for proteomics analysis | en_US |
| dc.type | Thesis | en |