A method to isolate the CTD of RNA Polymerase II for proteomics analysis
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Date
2014-12
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American English
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M.S.
Degree Year
2014
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Department of Biochemistry & Molecular Biology
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Indiana University
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Abstract
In an effort to advance the methodology in analyzing RNAPII protein-protein interaction network and to determine the role of the CTD in controlling RNAPII transcription, we devised a method to specifically isolate the CTD-associated and CTD-less RNAPII to identify the proteins that interact with both the CTD and the globular core of RNAPII using novel purification scheme coupled to quantitative proteomics.
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Indiana University-Purdue University Indianapolis (IUPUI)
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