Interspecies Comparison of Alveolar Bone Biology, Part I: Morphology and Physiology of Pristine Bone

dc.contributor.authorPilawski, I.
dc.contributor.authorTulu, U. S.
dc.contributor.authorTicha, P.
dc.contributor.authorSchüpbach, P.
dc.contributor.authorTraxler, H.
dc.contributor.authorXu, Q.
dc.contributor.authorPan, J.
dc.contributor.authorCoyac, B. R.
dc.contributor.authorYuan, X.
dc.contributor.authorTian, Y.
dc.contributor.authorLiu, Y.
dc.contributor.authorChen, J.
dc.contributor.authorErdogan, Y.
dc.contributor.authorArioka, M.
dc.contributor.authorArmaro, M.
dc.contributor.authorWu, M.
dc.contributor.authorBrunski, J. B.
dc.contributor.authorHelms, J. A.
dc.contributor.departmentOtolaryngology -- Head and Neck Surgery, School of Medicine
dc.date.accessioned2024-11-26T16:27:55Z
dc.date.available2024-11-26T16:27:55Z
dc.date.issued2021
dc.description.abstractIntroduction: Few interspecies comparisons of alveolar bone have been documented, and this knowledge gap raises questions about which animal models most accurately represent human dental conditions or responses to surgical interventions. Objectives: The objective of this study was to employ state-of-the-art quantitative metrics to directly assess and compare the structural and functional characteristics of alveolar bone among humans, mini pigs, rats, and mice. Methods: The same anatomic location (i.e., the posterior maxillae) was analyzed in all species via micro-computed tomographic imaging, followed by quantitative analyses, coupled with histology and immunohistochemistry. Bone remodeling was evaluated with alkaline phosphatase activity and tartrate-resistant acid phosphatase staining to identify osteoblast and osteoclast activities. In vivo fluorochrome labeling was used as a means to assess mineral apposition rates. Results: Collectively, these analyses demonstrated that bone volume differed among the species, while bone mineral density was equal. All species showed a similar density of alveolar osteocytes, with a highly conserved pattern of collagen organization. Collagen maturation was equal among mouse, rat, and mini pig. Bone remodeling was a shared feature among the species, with morphologically indistinguishable hemiosteonal appearances, osteocytic perilacunar remodeling, and similar mineral apposition rates in alveolar bone. Conclusions: Our analyses demonstrated equivalencies among the 4 species in a plurality of the biological features of alveolar bone. Despite contradictory results from older studies, we found no evidence for the superiority of pig models over rodent models in representing human bone biology. Knowledge transfer statement: Animal models are extensively used to evaluate bone tissue engineering strategies, yet there are few state-of-the-art studies that rigorously compare and quantify the factors influencing selection of a given animal model. Consequently, there is an urgent need to assess preclinical animal models for their predictive value to dental research. Our article addresses this knowledge gap and, in doing so, provides a foundation for more effective standardization among animal models commonly used in dentistry.
dc.eprint.versionFinal published version
dc.identifier.citationPilawski I, Tulu US, Ticha P, et al. Interspecies Comparison of Alveolar Bone Biology, Part I: Morphology and Physiology of Pristine Bone. JDR Clin Trans Res. 2021;6(3):352-360. doi:10.1177/2380084420936979
dc.identifier.urihttps://hdl.handle.net/1805/44738
dc.language.isoen_US
dc.publisherSage
dc.relation.isversionof10.1177/2380084420936979
dc.relation.journalJDR Clinical & Translational Research
dc.rightsPublisher Policy
dc.sourcePMC
dc.subjectAnimal models
dc.subjectBone loss
dc.subjectDental implant(s)
dc.subjectHydroxyapatite
dc.subjectMicro–computed tomography
dc.subjectRemodeling/ regeneration
dc.titleInterspecies Comparison of Alveolar Bone Biology, Part I: Morphology and Physiology of Pristine Bone
dc.typeArticle
ul.alternative.fulltexthttps://pmc.ncbi.nlm.nih.gov/articles/PMC8209842/
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