Understanding the Integrated Pathophysiological Role of a Moonlighting Protein in Lung Development

dc.contributor.advisorSchwarz, Margaret
dc.contributor.advisorTune, Johnathan
dc.contributor.authorLee, Dong Il
dc.contributor.otherKaplan, Mark
dc.contributor.otherBasile, David
dc.date.accessioned2019-08-28T16:37:23Z
dc.date.available2020-02-21T10:30:12Z
dc.date.issued2019-08
dc.degree.date2019en_US
dc.degree.discipline
dc.degree.grantorIndiana Universityen_US
dc.degree.levelPh.D.en_US
dc.descriptionIndiana University-Purdue University Indianapolis (IUPUI)en_US
dc.description.abstractSensing, integrating, and relaying signals from the environment through proteins, metabolites, and lipids to the lung are critical for proper development. Moonlighting proteins, such as AIMP1, are a unique subset that serves at least two independent physiological functions. Encoded by gene AIMP1, AIMP1 has two known functions: (1) C-terminus EMAP II domain of full-length AIMP1 can be secreted out of the cell to chemoattract myeloid cells; (2) intracellular full-length protein interacts with tRNA synthetases in protein translation. However, despite the linkage of protein expression levels of with several lung pathologies such as bronchopulmonary dysplasia (BPD), effectively targeting the protein encoded by AIMP1 has been a challenge due to poorly understood mechanisms. This thesis explores physiological, signaling, and immunological moonlighting mechanisms of first, the extracellular EMAP II then the intracellular AIMP1. Experiments utilize both in vitro and in vivo models, including a murine model of BPD and Cre-mediated exon-deletion knockout. Experimental results provide evidence that in the BPD model, EMAP II levels are elevated and sustained – first in bronchial epithelial cells then in macrophages. Mice exposed to sustained and elevated EMAP II protein levels resemble the BPD phenotype while neutralization partially rescued the phenotype, implying EMAP II as a potential therapeutic target against BPD. Results from studies exploring EMAP II’s signaling mechanism identify transient stimulation of JAK-STAT3 phosphorylation, commonly found in inflammation-resolving macrophages. In contrast, it induces unique transcriptional changes that are reversible both by JAK-STAT inhibitor and siRNA-mediated knockdown of Stat3. Studies using AIMP1 knockout mouse reveal a novel function for the intracellular AIMP1. AIMP1 knockout mice exhibited neonatal lethality with a respiratory distress phenotype, decreased type I alveolar cell expression, and disorganized bronchial epithelium, suggesting a role in lung maturation. In vitro experiments suggest that a portion of AIMP1 residing in the cell’s membrane interacts with various phosphatidylinositols and contributes toward F-actin deposition and assembly. Data from these experimental studies provide insight into how the various functions of the promiscuous AIMP1 gene affect lung development. These studies exemplify not only characterize novel moonlighting mechanisms of AIMP1, but also highlight the importance of characterizing moonlighting proteins to promote therapeutic preventions.en_US
dc.description.embargo2020-02-21
dc.identifier.urihttps://hdl.handle.net/1805/20662
dc.identifier.urihttp://dx.doi.org/10.7912/C2/2029
dc.language.isoen_USen_US
dc.subjectAIMP1/EMAP IIen_US
dc.subjectLungen_US
dc.subjectMacrophageen_US
dc.subjectPhysiologyen_US
dc.subjectSignalingen_US
dc.titleUnderstanding the Integrated Pathophysiological Role of a Moonlighting Protein in Lung Developmenten_US
dc.typeDissertation
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