INTERACTIONS OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS WITH TOBACCO TREATED STREPTOCOCCUS MUTANS

dc.contributor.authorGupta, Vinayak
dc.contributor.authorWindsor, L. Jack
dc.contributor.authorSong, Fengyu
dc.contributor.authorGregory, Richard L.
dc.date.accessioned2015-10-02T13:16:43Z
dc.date.available2015-10-02T13:16:43Z
dc.date.issued2013-04-05
dc.descriptionposter abstracten_US
dc.description.abstractStreptococcus mutans (S. mutans) is a major contributor to dental caries. Previous research has shown that there is a positive relationship between smoking and dental carries, however the pathway of S. mutans growth is not yet understood. Tobacco use affects the cardiovascular system and increases the rate of cardiovascular disease among smokers. However, the effects of tobacco on the endothelial cells that line blood vessels are not yet fully understood. Thus, the objective of this study was to examine some of the effects that a periodontal pathogen such as S. mutans treated with cigarette smoke condensate (CSC) and nicotine have on human umbilical vein endothelial cells (HUVEC’s). The S. mutans was grown at 37°C and then the planktonic cells were harvested, washed with saline, and then killed with formaldehyde. To standardize the samples, they were diluted to the same OD at 600nm wavelength using a spectroscope. The HUVEC were cultured in Endothelial Basal Medium-2 and plated in 12 well plates and exposed to the P. gingivalis cells and supernatants and after 72 hours, lactate dehydrogenase (LDH) assays will be used to cytotoxicity. Non-toxic amounts of the cells and supernatants will then be used to treat HUVEC cells for 72 hours before the media is collected and analyzed for cytokine/growth factor expression by protein arrays. Second messenger signaling pathways will be analyzed with ERK and JNK antagonists and agonists to determine the pathway of up regulation of S. mutans. A better understanding of the detrimental effects that tobacco has on the underlining causes of periodontal disease can advance the quest of controlling the disease.en_US
dc.identifier.citationGupta, Vinayak, L. Jack Windsor, Fengyu Song, and Richard Gregory. (2013, April 5). INTERACTIONS OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS WITH TOBACCO TREATED STREPTOCOCCUS MUTANS. Poster session presented at IUPUI Research Day 2013, Indianapolis, Indiana.en_US
dc.identifier.urihttps://hdl.handle.net/1805/7144
dc.language.isoen_USen_US
dc.publisherOffice of the Vice Chancellor for Researchen_US
dc.subjecthuman umbilical vein endothelial cellsen_US
dc.subjecttobacco treated streptococcus mutansen_US
dc.subjectdental cariesen_US
dc.subjectcigarette smoke condensateen_US
dc.subjectnicotineen_US
dc.titleINTERACTIONS OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS WITH TOBACCO TREATED STREPTOCOCCUS MUTANSen_US
dc.typePosteren_US
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