INTERACTIONS OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS WITH TOBACCO TREATED STREPTOCOCCUS MUTANS

Date
2013-04-05
Language
American English
Embargo Lift Date
Department
Committee Members
Degree
Degree Year
Department
Grantor
Journal Title
Journal ISSN
Volume Title
Found At
Office of the Vice Chancellor for Research
Can't use the file because of accessibility barriers? Contact us with the title of the item, permanent link, and specifics of your accommodation need.
Abstract

Streptococcus mutans (S. mutans) is a major contributor to dental caries. Previous research has shown that there is a positive relationship between smoking and dental carries, however the pathway of S. mutans growth is not yet understood. Tobacco use affects the cardiovascular system and increases the rate of cardiovascular disease among smokers. However, the effects of tobacco on the endothelial cells that line blood vessels are not yet fully understood. Thus, the objective of this study was to examine some of the effects that a periodontal pathogen such as S. mutans treated with cigarette smoke condensate (CSC) and nicotine have on human umbilical vein endothelial cells (HUVEC’s). The S. mutans was grown at 37°C and then the planktonic cells were harvested, washed with saline, and then killed with formaldehyde. To standardize the samples, they were diluted to the same OD at 600nm wavelength using a spectroscope. The HUVEC were cultured in Endothelial Basal Medium-2 and plated in 12 well plates and exposed to the P. gingivalis cells and supernatants and after 72 hours, lactate dehydrogenase (LDH) assays will be used to cytotoxicity. Non-toxic amounts of the cells and supernatants will then be used to treat HUVEC cells for 72 hours before the media is collected and analyzed for cytokine/growth factor expression by protein arrays. Second messenger signaling pathways will be analyzed with ERK and JNK antagonists and agonists to determine the pathway of up regulation of S. mutans. A better understanding of the detrimental effects that tobacco has on the underlining causes of periodontal disease can advance the quest of controlling the disease.

Description
poster abstract
item.page.description.tableofcontents
item.page.relation.haspart
Cite As
Gupta, Vinayak, L. Jack Windsor, Fengyu Song, and Richard Gregory. (2013, April 5). INTERACTIONS OF HUMAN UMBILICAL VEIN ENDOTHELIAL CELLS WITH TOBACCO TREATED STREPTOCOCCUS MUTANS. Poster session presented at IUPUI Research Day 2013, Indianapolis, Indiana.
ISSN
Publisher
Series/Report
Sponsorship
Major
Extent
Identifier
Relation
Journal
Source
Alternative Title
Type
Poster
Number
Volume
Conference Dates
Conference Host
Conference Location
Conference Name
Conference Panel
Conference Secretariat Location
Version
Full Text Available at
This item is under embargo {{howLong}}