Manufacturing Dendritic Cells for Immunotherapy: Monocyte Enrichment
| dc.contributor.author | Hopewell, Emily L. | |
| dc.contributor.author | Cox, Cheryl | |
| dc.contributor.department | Medical and Molecular Genetics, School of Medicine | en_US |
| dc.date.accessioned | 2020-03-31T13:07:41Z | |
| dc.date.available | 2020-03-31T13:07:41Z | |
| dc.date.issued | 2020-01-15 | |
| dc.description.abstract | Dendritic cells play a key role in activation of the immune system as potent antigen-presenting cells. This pivotal position, along with the ability to generate dendritic cells from monocytes and ready uptake of antigen, makes them an intriguing vehicle for immunotherapy for a variety of indications. Since the first reported trial using dendritic cells in 1995, they have been used in trials all over the world for a plethora of indications. Monocyte-derived dendritic cells are generated from whole blood or apheresis products by culturing enriched monocytes in the presence of interleukin (IL)-4 and granulocyte-macrophage colony-stimulating factor (GM-CSF). A variety of methods can be used for enrichment of monocytes for generation of clinical-grade dendritic cells and are summarized herein. | en_US |
| dc.eprint.version | Final published version | en_US |
| dc.identifier.citation | Hopewell, E. L., & Cox, C. (2020). Manufacturing Dendritic Cells for Immunotherapy: Monocyte Enrichment. Molecular therapy. Methods & clinical development, 16, 155–160. https://doi.org/10.1016/j.omtm.2019.12.017 | en_US |
| dc.identifier.uri | https://hdl.handle.net/1805/22433 | |
| dc.language.iso | en_US | en_US |
| dc.publisher | Elsevier | en_US |
| dc.relation.isversionof | 10.1016/j.omtm.2019.12.017 | en_US |
| dc.relation.journal | Molecular Therapy | en_US |
| dc.rights | Attribution-NonCommercial-NoDerivatives 4.0 International | * |
| dc.rights.uri | http://creativecommons.org/licenses/by-nc-nd/4.0/ | * |
| dc.source | PMC | en_US |
| dc.subject | Dendritic Cells | en_US |
| dc.subject | Immunotherapy | en_US |
| dc.subject | Monocytes | en_US |
| dc.subject | GM-CSF | en_US |
| dc.title | Manufacturing Dendritic Cells for Immunotherapy: Monocyte Enrichment | en_US |
| dc.type | Article | en_US |