Sensory innervation in the prostate and a role for calcitonin gene-related peptide in prostatic epithelial proliferation

dc.contributor.authorXia, Hanyu
dc.contributor.authorJerde, Travis J.
dc.contributor.authorFehrenbacher, Jill C.
dc.contributor.departmentPharmacology and Toxicology, School of Medicine
dc.date.accessioned2025-02-25T09:25:49Z
dc.date.available2025-02-25T09:25:49Z
dc.date.issued2024-12-18
dc.description.abstractIntroduction: The prostate is densely innervated like many visceral organs and glands. However, studies to date have focused on sympathetic and parasympathetic nerves and little attention has been given to the presence or function of sensory nerves in the prostate. Recent studies have highlighted a role for sensory nerves beyond perception of noxious stimuli, as anterograde release of neuropeptides from sensory nerves can affect vascular tone and local immune responses. Methods: To identify the degree of sensory innervation in the prostate, we utilized state-of-the-art tissue clearing and microscopy to visualize sensory innervation in the different lobes of the mouse prostate. To determine whether sensory nerves have a role in regulating proliferation within the prostate, we used an intersectional genetic and toxin approach to ablate peptidergic sensory nerves systemically. Results: We found that sensory neurons are abundant in the prostate both in nerve bundles along the vasculature and as independent nerve fibers wrapped around prostatic acini in a net-like fashion. In addition to the dense innervation of the prostate, we found that Calca haploinsufficiency, the genotype control for our intersectional ablation model, results in a diminished level of Ki67 staining in the stromal compartment of the dorsal lobe and a diminishing Ki67 trend in other lobes. Discussion: These findings suggest that sensory neurons might have developmental or homeostatic effects within the prostate. Further studies are warranted to assess the role of sensory neurons and the sensory neuropeptides on prostatic development and on proliferation in the presence of pro-inflammatory stimuli such as bacterial infection or tumor cells.
dc.eprint.versionFinal published version
dc.identifier.citationXia H, Jerde TJ, Fehrenbacher JC. Sensory innervation in the prostate and a role for calcitonin gene-related peptide in prostatic epithelial proliferation. Front Mol Neurosci. 2024;17:1497735. Published 2024 Dec 18. doi:10.3389/fnmol.2024.1497735
dc.identifier.urihttps://hdl.handle.net/1805/45990
dc.language.isoen_US
dc.publisherFrontiers Media
dc.relation.isversionof10.3389/fnmol.2024.1497735
dc.relation.journalFrontiers in Molecular Neuroscience
dc.rightsAttribution 4.0 Internationalen
dc.rights.urihttps://creativecommons.org/licenses/by/4.0
dc.sourcePMC
dc.subjectProstate
dc.subjectProstate innervation
dc.subjectSensory nerves
dc.subjectCalcitonin gene-related peptide
dc.subjectTissue clearing
dc.subject3D imaging
dc.subjectCell proliferation
dc.subjectDeep learning image segmentation
dc.titleSensory innervation in the prostate and a role for calcitonin gene-related peptide in prostatic epithelial proliferation
dc.typeArticle
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